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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Influence of seed priming with iron and/or zinc in the nucleolar activity and protein content of bread wheat
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Influence of seed priming with iron and/or zinc in the nucleolar activity and protein content of bread wheat

机译：种子灌注用铁和/或锌在面包小麦核仁活性和蛋白质含量的影响

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Seed priming with iron (Fe) and/or zinc (Zn) can overcome the reduced availability of these micronutrients in soils and crops, but suitable dosages should be predetermined. Nucleolus responds to stress, such as cytotoxicity, with alterations perceivable by cytogenetic analyses. This work intends to study how seed priming with Fe and/or Zn affects the nucleolar activity in roots and the total soluble protein content in the flour of bread wheat cv. JordAo'. Seven priming treatments with 0mgL(-1) to 8mgL(-1) of Fe and/or Zn were performed. In all treatments, each metaphase cell presented a maximum of six nucleolar organizer regions positively stained with silver nitrate (Ag-NORs). Also, a maximum number of six nucleoli per nucleus were observed in all treatments, except in the hydroprimed seeds (used as control) that showed a maximum of five nucleoli, probably due to nucleolar fusion. Irregular interphases were frequent in treatments with the highest dosage of micronutrients (8mgL(-1) Fe and/or 8mgL(-1) Zn). The nucleolar area reduced (p<0.001) as the number of nucleoli increased, and it was lower in treatments with a combination of Fe and Zn. However, the combinations of Fe and Zn showed the highest concentrations of total soluble protein (p0.001). Although a reduced nucleolar area represents low ribosomal RNA gene transcription and ribosomal production, the significant increase of the number of nucleoli in the seeds primed with Fe and Zn enhanced the total soluble protein content as compared to the hydroprimed seeds (control) probably due to an increase of nucleolar surface-to-volume ratio that improved the protein synthesis. Overall, this work revealed that priming of bread wheat seeds with suited dosages of Fe and Zn can improve the nutritional value of flour, and the nucleolar number, morphology, and area can be useful biomarkers in cytotoxicity studies.
机译：用铁（Fe）和/或锌（Zn）的种子引发可以克服土壤和作物中这些微量营养素的降低的可用性，但应该预先确定合适的剂量。核仁响应应激，例如细胞毒性，随着细胞遗传学分析可感知的改变。这项工作旨在研究用Fe和/或Zn的种子引发如何影响根部的根系中的核仁活性和面包小麦CV面粉中的总可溶性蛋白质含量。 jordao'。进行七种具有0mgL（-1）至8mgL（-1）的Fe和/或Zn的初步处理。在所有治疗中，每个中期细胞呈现最多六个核核组织者区域，用硝酸银（Ag-nors）呈正染色。此外，在所有治疗中观察到每种细胞核的最大六个核仁，除了在核磁融合中显示最多五个核仁的水化种子（用作对照）。在具有最高剂量的微量营养素（8mgL（-1）Fe和/或8mgL（-1）Zn）中的处理中频繁频繁频繁。随着核仁的数量增加，核仁区域降低（p <0.001），并且在具有Fe和Zn的组合的处理中较低。然而，Fe和Zn的组合显示出最高浓度的总可溶性蛋白质（P0.001）。虽然还原的核仁区域代表低核糖体RNA基因转录和核糖体产生，但与加氢种子（对照）相比，用Fe和Zn的种子中的种子中核仁数量的显着增加增强了可能导致的提高蛋白质合成的核仁表面对体积比。总体而言，这项工作透露，面包小麦种子具有适当剂量的Fe和Zn的引发可以改善面粉的营养价值，核仁数，形态和面积可以是细胞毒性研究中有用的生物标志物。

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