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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Can exposure to neem oil affect the spermatogenesis of predator Ceraeochrysa claveri?
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Can exposure to neem oil affect the spermatogenesis of predator Ceraeochrysa claveri?

机译：可以接触雷姆油影响捕食者Ceraeochrysa Claveri的精子发生吗？

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摘要

Novel biological control methods and integrated pest management strategies are basic requirements for the development of sustainable agriculture. As a result, there is a growing demand for research on the use of plant extracts and natural enemies such as the green lacewing, Ceraeochrysa claveri, as natural pest control methods. Studies have shown that although natural compounds such as neem oil (Azadirachta indica) are effective as pest control strategies, they also cause sublethal effects on nontarget insects, such as C. claveri. The aim of this study was to examine the effects of neem oil on C. claveri testes. C. claveri larvae were fed Diatraea saccharalis eggs, which were pretreated with 0.5%, 1%, and 2% neem oil. Testes were collected from larvae, pupae, and adults and analyzed using light and electron (transmission and scanning) microscopy. Changes in cellular stress and possible cell death were also determined by TUNEL assay and the marker HSP-70. The results showed that neem oil affects the organization and distribution of cysts in the testes and the normal sequence of cyst development, causing a delay in spermatogenesis in the testes of treated insects. Tests for cellular stress and DNA fragmentation indicated there was no cellular alteration in the treated groups. Although neem oil does not induce cell death or changes in HSP-70 expression, this biopesticide negatively impacts the process of spermatogenesis and could decrease the perpetuation of this species in the agroecosystem, indicating that the use of neem oil in association with green lacewings as a biological control should be carefully evaluated.
机译：新型生物控制方法和综合害虫管理策略是可持续农业发展的基本要求。因此，对使用植物提取物和天然敌人如绿色花草植物，Ceraeochrysa Claveri的使用，越来越大的需求，作为天然害虫控制方法。研究表明，尽管诸如雷维尔（Azadirachta indica）的天然化合物是有效的作为害虫控制策略，但它们也对Nontarget昆虫（例如C.Claveri）造成致重骨影响。本研究的目的是检测雷维尔睾丸睾丸睾丸对雷姆油的影响。 C. Claveri幼虫被喂食DiaTraea Saccharalis卵，用0.5％，1％和2％的Neem油预处理。从幼虫，蛹和成人收集睾丸，并使用光和电子（传输和扫描）显微镜分析。细胞应激和可能的细胞死亡的变化也是通过TUNEL测定和标记HSP-70测定的。结果表明，新泻油影响睾丸中囊肿的组织和分布和正常囊肿发育序列，导致治疗昆虫睾丸中的精子发生。细胞应激和DNA碎片的试验表明治疗组中没有细胞改变。虽然Neem油不会诱导细胞死亡或HSP-70表达的变化，但这种生物农药对精子发生的过程产生负面影响，并且可以降低农业生物系统中这种物种的永久性，表明使用Neem OIN与绿色花边的使用应仔细评估生物控制。

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