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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Utilisation of Toluidine blue O pH 4.0 and histochemical inferences in plant sections obtained by free-hand
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Utilisation of Toluidine blue O pH 4.0 and histochemical inferences in plant sections obtained by free-hand

机译：利用甲苯胺蓝o pH4.0和通过释放植物部分的组织化学推论

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摘要

For the observation of plant material under a light microscope, free-hand sectioning is usually the fastest and most practical procedure. The staining of the obtained cuts is a crucial step and the action mechanism of the dye is variable. Toluidine blue O is a cationic dye whose metachromatic potential for free-hand sections has long been known. Thus, this work aimed at analysing the performance of Toluidine blue O for this type of sectioning with a systematic and comparative approach. The action of Toluidine blue O pH 4.0 was tested for both fresh and previously clarified free-hand sections from leaves of different species of plant. These sections were compared with sections embedded in glycol-methacrylate resin stained with the same dye or submitted to different histochemical tests. The fresh sections obtained by free-hand and stained with Toluidine blue O permitted the accomplishment of histochemical inferences, with the advantage of maintaining the natural colours from the tissues. The previously clarified sections presented the advantage of having a clearer image, allowing a better cellular delimitation. We concluded that Toluidine blue O is very informative for sections of plant material obtained by free-hand, being, therefore, a procedure that is fast, inexpensive and with excellent results.
机译：为了在光学显微镜下观察植物材料，自由切割通常是最快，最实用的程序。所得切口的染色是关键步骤，染料的作用机理是可变的。甲苯胺蓝o是一种阳离子染料，其自由段的相位是已知的。因此，这种作品旨在通过系统和比较方法分析这种类型的切片的甲苯胺蓝o的性能。对来自不同种植叶片的新鲜和先前澄清的自由切片测试了甲苯胺蓝o pH 4.0的作用。将这些部分与嵌入的乙二醇 - 甲基丙烯酸酯树脂中的截面进行比较，或者用相同的染料染色或提交给不同的组织化学测试。通过释放和用甲苯胺蓝染色获得的新鲜部分允许完成组织化学推论，其优点是维持来自组织的自然色彩。先前澄清的部分呈现了具有更清晰的图像的优点，允许更好的蜂窝划界。我们得出结论，甲苯胺蓝o对于通过释放，因此植物材料的植物材料部分非常富有了信息性，因此是一种快速，廉价且结果优异的程序。

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