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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Aminooxyacetic acid (AOA), inhibitor of 1-aminocyclopropane-1-carboxilic acid (ACC) synthesis, suppresses self-incompatibility-induced programmed cell death in self-incompatible Petunia hybrida L. pollen tubes
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Aminooxyacetic acid (AOA), inhibitor of 1-aminocyclopropane-1-carboxilic acid (ACC) synthesis, suppresses self-incompatibility-induced programmed cell death in self-incompatible Petunia hybrida L. pollen tubes

机译:氨基氧基乙酸(AOA),1-氨基环丙烷-1-羧酸(ACC)合成的抑制剂,抑制了自我不相容性诱导的自我不相容的Petunia Hybrida L.花粉管中的编程细胞死亡

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摘要

Self-incompatibility (SI) is genetically determined reproductive barrier preventing inbreeding and thereby providing the maintenance of plant species diversity. At present, active studies of molecular bases of SI mechanisms are underway. S-RNAse-based SI in Petunia hybrida L. is a self-/non-self recognition system that allows the pistil to reject self pollen and to accept non-self pollen for outcrossing. In the present work, using fluorescent methods including the TUNEL method allowed us to reveal the presence of markers of programmed cell death (PCD), such as DNA fragmentation, in growing in vivo petunia pollen tubes during the passage of the SI reaction. The results of statistical analysis reliably proved that PCD is the factor of S-RNAse-based SI. It was found that preliminary treatment before self-pollination of stigmas of petunia self-incompatible line with aminooxyacetic acid (AOA), inhibitor of ACC synthesis, led to stimulation of pollen tubes growth when the latter did not exhibit any hallmarks of PCD. These data argue in favor of assumption that ethylene controls the passage of PCD in incompatible pollen tubes in the course of S-RNAse-based SI functioning. The involvement of the hormonal regulation in SI mechanism in P. hybrida L. is the finding observed by us for the first time.
机译:自我不相容性(Si)是遗传确定的生殖障碍防止近亲繁殖,从而提供植物物种多样性的维持。目前,正在进行中,对Si机制的分子碱进行积极研究。基于S-RNase的Si在培养皿Hybrida L.是一种自我/非自我识别系统,允许雌蕊抑制自我花粉并接受非自我花粉以进行折叠。在本作工作中,使用包括TUNEL方法的荧光方法使我们能够揭示编程细胞死亡(PCD)的标志物,例如DNA碎片,在SI反应通过体内培养的花粉管中生长。统计分析结果可靠地证明了PCD是基于S-RNase的Si的因子。结果发现,在氨基氧基乙酸(AOA)中,氨基氧基乙酸(AOA),ACC合成抑制剂的初步处理,当后者没有表现出任何PCD的任何标志时,导致花粉管的刺激。这些数据有利于假设乙烯在基于S-RNase的SI功能过程中控制PCD在不相容的花粉管中的通过。激素调节在P. hybrida L中的Si机制中的累积是我们第一次观察到的发现。

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