Overexpression of a thaumatin-like protein gene from Vitis amurensis improves downy mildew resistance in Vitis vinifera grapevine

He Rongrong; Wu Jiao; Zhang Yali; Aguero Cecilia B.; Li Xinlong; Liu Shaoli; Wang Chaoxia; Walker M. Andrew; Lu Jiang

首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Overexpression of a thaumatin-like protein gene from Vitis amurensis improves downy mildew resistance in Vitis vinifera grapevine

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Overexpression of a thaumatin-like protein gene from Vitis amurensis improves downy mildew resistance in Vitis vinifera grapevine

机译：来自血管腺炎的血清素样蛋白基因的过表达提高了血管血管葡萄葡萄树中的稳定效力

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Downy mildew is a highly destructive disease in grapevine production. A gene encoding pathogenesis-related (PR) thaumatin-like protein was isolated from the downy mildew-resistant grapevine "Zuoshan-1," a clonal selection from wild Vitis amurensis Rupr. The predicted thaumatin-like protein (VaTLP) has 225 amino acids and it is acidic, with a calculated isoelectric point of 4.8. The full length of the VaTLP gene was transformed into somatic embryogenic calli of V. vinifera 'Thompson Seedless' via Agrobacterium tumefaciens. Real-time RT-PCR confirmed that the VaTLP gene was expressed at a high level in the transgenic grapevines. Improved resistance of the transgenic lines against downy mildew was evaluated using leaf disks and whole plants inoculated with Plasmopara viticola, the pathogen causing grapevine downy mildew disease. Bioassay of the pathogen showed that both hyphae growth and asexual reproduction were inhibited significantly among the transgenic plants. Histological analysis also confirmed this disease resistance by demonstrating the inhibition and malformation of hyphae development in leaf tissue of the transgenic plants. These results indicated that the accumulation of VaTLP could enhance resistance to P. viticola in transgenic 'Thompson Seedless' grapevines.

机译：霜霉病是葡萄生产中的一种高度破坏性疾病。从粪便抗性葡萄“Zuoshan-1”中分离出疾病相关（Pr）血清蛋白样蛋白的基因与野生血管血管rupr的克隆选择分离出疾病抗性葡萄葡萄族“祖山-1”。预测的硫甜桃样蛋白（Vatlp）具有225个氨基酸，并且是酸性的，计算出的等电点为4.8。通过土壤杆菌突发，VatlP基因的全长转化为V.Vinifera'Thompson无籽'的体细胞胚胎愈伤组织。实时RT-PCR证实VatlP基因在转基因葡萄树中的高水平表达。使用叶片磁盘和血浆伏族伏替洛拉的叶片和整株植物来评估转基因株对剧本霉菌的改善的抗性，导致葡萄瓣剧本疾病的病原体。病原体的生物测定表明，在转基因植物中显着抑制了菌丝生长和无性繁殖。组织学分析还通过证明转基因植物的叶组织中菌丝发育的抑制和畸形来证实这种疾病抵抗力。这些结果表明，Vatlp的积累可以增强转基因“汤普森无籽”葡萄藤中对viticola的抗性。

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来源
《Protoplasma: An International Journal of Cell Biology》 |2017年第4期|共11页
作者
He Rongrong; Wu Jiao; Zhang Yali; Aguero Cecilia B.; Li Xinlong; Liu Shaoli; Wang Chaoxia; Walker M. Andrew; Lu Jiang;
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作者单位

China Agr Univ Coll Food Sci &

Nutr Engn Viticulture &

Enol Program Beijing 100083 Peoples R China;

China Agr Univ Coll Food Sci &

Nutr Engn Viticulture &

Enol Program Beijing 100083 Peoples R China;

China Agr Univ Coll Food Sci &

Nutr Engn Viticulture &

Enol Program Beijing 100083 Peoples R China;

Univ Calif Davis Dept Viticulture &

Enol Davis CA 95616 USA;

China Agr Univ Coll Food Sci &

Nutr Engn Viticulture &

Enol Program Beijing 100083 Peoples R China;

China Agr Univ Coll Food Sci &

Nutr Engn Viticulture &

Enol Program Beijing 100083 Peoples R China;

China Agr Univ Coll Food Sci &

Nutr Engn Viticulture &

Enol Program Beijing 100083 Peoples R China;

Univ Calif Davis Dept Viticulture &

Enol Davis CA 95616 USA;

China Agr Univ Coll Food Sci &

Nutr Engn Viticulture &

Enol Program Beijing 100083 Peoples R China;

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原文格式 PDF
正文语种 eng
中图分类细胞形态学;
关键词
Grapevine; Downy mildew; Resistance; Thaumatin-like protein; Transformation;

机译：葡萄树;柔软的霉菌;抗性;甲磺汀样蛋白质;转化;

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专利

1. Overexpression of a thaumatin-like protein gene from Vitis amurensis improves downy mildew resistance in Vitis vinifera grapevine [J] . He Rongrong, Wu Jiao, Zhang Yali, Protoplasma: An International Journal of Cell Biology . 2017,第4期

机译：来自血管腺炎的血清素样蛋白基因的过表达提高了血管血管葡萄葡萄树中的稳定效力
2. Construction of a reference linkage map of Vitis amurensis and genetic mapping of Rpv8, a locus conferring resistance to grapevine downy mildew [J] . Paule Blasi, Sophie Blanc, Sabine Wiedemann-Merdinoglu, Theoretical and Applied Genetics . 2011,第1期

机译：葡萄的参考连锁图谱的构建和Rpv8的遗传图谱，Rpv8是赋予葡萄霜霉病抗性的一个位点
3. Vitis vinifera VvNPR1.1 is the functional ortholog of AtNPR1 and its overexpression in grapevine triggers constitutive activation of PR genes and enhanced resistance to powdery mildew [J] . Le Henanff G., Farine S., Kieffer-Mazet F., Planta: An International Journal of Plant Biology . 2011,第2期

机译：葡萄（Vitis vinifera）VvNPR1.1是AtNPR1的功能直系同源物，其在葡萄中的过表达引发PR基因的组成型激活并增强了对白粉病的抵抗力
4. Population Heredity Trend of Several Traits from the Cross 'Red Globe' (Vitis Vinifera L.) x 'Shuangyou' (Vitis Amurensis Rupr.) [C] . GUO Yin-shan, LIN Hong, LIU Zhendong, Conference on key technology of horticulture . 2011

机译：十字“红色地球”（Vitis Vinifera L.）x“双有”（Vitis Amurensis Rupr。）几种性状的群体遗传趋势
5. Changes in gene expression in response to abiotic stress in grapevine (Vitis vinifera). [D] . Tattersall, Elizabeth Ann Russell. 2006

机译：响应非生物胁迫（葡萄）的基因表达变化。
6. Historical Introgression of the Downy Mildew Resistance Gene Rpv12 from the Asian Species Vitis amurensis into Grapevine Varieties [O] . Silvia Venuti, Dario Copetti, Serena Foria, -1

机译：亚洲种葡萄的霜霉病抗性基因Rpv12的历史渗入到葡萄品种中
7. Tartós rezisztencia szőlőben: markerekre alapozott génpiramidálás és két, Vitis vinifera eredetű lisztharmat rezisztenciagén összehasonlítása = Durable resistance in grapevine: MAS- based gene pyramiding and comparison of two powdery mildew resistance genes of Vitis vinifera origin [O] . Veres Anikó 2011

机译：葡萄中的持久抗性：基于标记的基因金字塔化和酿酒葡萄的两个白粉病抗性基因的比较=葡萄的持久抗性：基于MAS的基因金字塔化和酿酒葡萄来源的两个白粉病抗性基因的比较

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Overexpression of a thaumatin-like protein gene from Vitis amurensis improves downy mildew resistance in Vitis vinifera grapevine

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