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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Histone H3 and H4 acetylation patterns are more dynamic than those of DNA methylation in Brachypodium distachyon embryos during seed maturation and germination
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Histone H3 and H4 acetylation patterns are more dynamic than those of DNA methylation in Brachypodium distachyon embryos during seed maturation and germination

机译：组蛋白H3和H4乙酰化图案比种子成熟和萌发期间的沸腾毒液胚胎中的DNA甲基化更加动态

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摘要

The transition of seeds from a dry to a meta-bolically active state requires significant changes in both the spatial and temporal patterns of gene expression, and this transcriptional reprogramming involves various modifications of the chromatin structure. There are several factors that can greatly influence the structure of chromatin, one of which is the chemical modifications of histone proteins and DNA itself. In this study, we analysed the distribution of three epigenetic markers, i.e. acetylation of histone H4 (H4K16ac) and histone H3 (H3K18ac) as well as DNA methylation (5mC) in Brachypodium distachyon embryos during the four stages of seed development-maturation, desiccation (quiescence), imbibition and germination. Our results indicate that both H4K16ac and H3K18ac are at a very high level in embryos during seed imbibition, but that the patterns of DNA methylation are considerably more stable in embryos during seed development.
机译：种子从干燥到荟牙活性状态的转变需要基因表达的空间和时间模式的显着变化，并且该转录重编程涉及染色质结构的各种修饰。 有几个因素可以大大影响染色质的结构，其中一个是组蛋白蛋白和DNA本身的化学修饰。 在这项研究中，我们分析了三种表观遗传标记的分布，即组蛋白H4（H4K16AC）和组蛋白H3（H3K18Ac）的乙酰化在种子发育成熟的四个阶段的血管缩孔胚胎中的DNA甲基化（5MC），干燥 （静止），吸收和发芽。 我们的结果表明，H4K16AC和H3K18AC在种子吸收期间胚胎中的非常高，但在种子发育过程中，DNA甲基化的模式在胚胎中相当稳定。

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