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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Isolation and functional characterization of 3-phosphoglycerate dehydrogenase involved in salt responses in sugar beet
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Isolation and functional characterization of 3-phosphoglycerate dehydrogenase involved in salt responses in sugar beet

机译：甜菜盐响应中涉及的3-磷酸糖脱氢酶的分离与功能表征

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摘要

The present study investigated the significance of serine biosynthetic genes for salt stress in sugar beet (Beta vulgaris). We isolated a total of four genes, two each encoding D-3-phosphoglycerate dehydrogenase (BvPGDHa and BvPGDHb) and serine hydroxymethyl transferase (BvSHMTa and BvSHMTb). mRNA transcriptional expression for BvPGDHa was significantly enhanced under salt stress conditions in both leaves and roots of sugar beet, whereas it was reduced for BvPGDHb. On the other hand, BvSHMTa was expressed transiently in leaves and roots under salt stress, whereas expression level of BvSHMTb was not altered. PGDH activity was high in storage root. After salt stress, PGDH activity was increased in leaf, petiole, and root. Recombinant proteins were expressed in Escherichia coli. The K (m) values for 3-phosphoglycerate in PGDHa and PGDHb were 1.38 and 2.92 mM, respectively. The findings suggest that BvPGDHa and BvSHMTa play an important role during salt stress in sugar beet.
机译：本研究研究了甜菜（甜菜碱）中钠盐胁迫的盐胁迫的重要性。 我们隔离共有四种基因，两个编码D-3-磷酸糖脱氢酶（BVPGDHA和BVPGDHB）和丝氨酸羟甲基转移酶（BVSHMTA和BVSHMTB）。 BVPGDHA的mRNA转录表达在甜菜和根部的盐胁迫条件下显着提高，而BVPGDH的盐胁迫条件明显增强。 另一方面，BVSHMTA在盐胁迫下瞬时表达，而BVSHMTB的表达水平未被改变。 PGDH活性在储存根中很高。 盐胁迫后，叶片，叶柄和根部的PGDH活性增加。 重组蛋白在大肠杆菌中表达。 PGDHA和PGDHB中的3-磷酸糖的K（m）值分别为1.38和2.92mm。 研究结果表明，BVPGDHA和BVSHMTA在甜菜中的盐胁迫期间发挥着重要作用。

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