...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Karyotype reshufflings of Festuca pratensis x Lolium perenne hybrids
【24h】

Karyotype reshufflings of Festuca pratensis x Lolium perenne hybrids

机译：Festuca的核型Reshufflings Pratensis X Lolium Perenne杂种

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Many different processes have an impact on the shape of plant karyotype. Recently, cytogenetic examination of Lolium species has revealed the occurrence of spontaneous fragile sites (FSs) associated with 35S rDNA regions. The FSs are defined as the chromosomal regions that are sensitive to forming gaps or breaks on chromosomes. The shape of karyotype can also be determined by interstitial telomeric sequences (ITSs), what was recognized for the first time in this paper in chromosomes of Festuca pratensis x Lolium perenne hybrids. Both FSs and ITSs can contribute to genome instabilities and chromosome rearrangements. To evaluate whether these cytogenetic phenomena have an impact on karyotype reshuffling observed in Festuca x Lolium hybrids, we examined F-1 F. pratensis x L. perenne plants and generated F-2-F-9 progeny by fluorescent in situ hybridization (FISH) using rDNA sequences, telomere and centromere probes, as well as by genomic in situ hybridization (GISH). Analyses using a combination of FISH and GISH revealed that intergenomic rearrangements did not correspond to FSs but overlapped with ITSs for several analyzed genotypes. It suggests that internal telomeric repeats can affect the shape of F. pratensis x L. perenne karyotypes. However, other factors that are involved in rearrangements and have a more crucial impact could exist, but they are still unknown.
机译：许多不同的过程对植物核型的形状产生影响。最近，对黑发物种的细胞遗传学检查揭示了与35s RDNA区域相关的自发脆弱位点（FSS）的发生。 FSS定义为对形成间隙或染色体上的破裂敏感的染色体区域。核型的形状也可以通过间质聚粒序列（ITS）来确定，本文首次在Festuca Pratensis X Lolium Perenne杂交种中首次认可的内容。 FSS及其既可以有助于基因组稳定性和染色体重排。为了评估这些细胞遗传学现象是否对在Festuca X Loliumbrds中观察到的核型重新洗脱，我们检查了F-1 F.Pratensis X L. Perenne植物并通过荧光原位杂交（鱼）产生F-2-F-9后代使用RDNA序列，端粒和符号探针以及原位杂交（GISH）的基因组。使用鱼和GISH的组合分析显示，骨髓组重排与FSS不对应，但与其有几种分析的基因型重叠。它表明内部端粒体重复会影响F.Pratensis X L. perenne核型的形状。然而，可能存在涉及重排和更为关键影响的其他因素，但它们仍然未知。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号