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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Evaluation of heme oxygenase 1 (HO 1) in Cd and Ni induced cytotoxicity and crosstalk with ROS quenching enzymes in two to four leaf stage seedlings of Vigna radiata
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Evaluation of heme oxygenase 1 (HO 1) in Cd and Ni induced cytotoxicity and crosstalk with ROS quenching enzymes in two to four leaf stage seedlings of Vigna radiata

机译：在CD和Ni诱导的细胞毒性和串扰中的血红素氧酶1（HO 1）评价与豇豆菌的两至四个叶阶段幼苗中的rOS猝灭酶诱导细胞毒性和串扰

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摘要

Research on heme oxygenase in plants has received consideration in recent years due to its several roles in development, defense, and metabolism during various environmental stresses. In the current investigation, the role of heme oxygenase (HO) 1 was evaluated in reducing heavy metal (Cd and Ni) uptake and alleviating Cd and Ni toxicity effects in the hydroponically grown seedlings of Vigna radiata var. PDM 54. Seedlings were subjected to Cd- and Ni-induced oxidative stress independently at different concentrations ranging from 10 to 100 mu M. After 96 h (fourth day) of treatment, the stressed plants were harvested to study the cellular homeostasis and detoxification mechanism by examining the growth, stress parameters (LPX, H2O2 content), and non-enzymatic and enzymatic parameters (ascorbate peroxidase (APX), guaicol peroxidase (GPX), and catalase (CAT)) including HO 1. At 50 mu M CdCl2 and 60 mu M NiSO4, HO 1 activity was found to be highest in leaves which were 1.39 and 1.16-fold, respectively. The greatest HO 1 activity was reflected from the reduction of H2O2 content at these metal concentrations (50 mu M CdCl2 and 60 mu M NiSO4) which is correlated with the increasing activity of other antioxidant enzymes (CAT, APX). Thus, HO 1 works within a group that generates the defense machinery for the plant's survival by scavenging ROS which is confirmed by a time-dependent study. Hence, it is concluded that seedlings of V. radiata were more tolerant towards metal-induced oxidative stress in which HO 1 is localized in its residential area (plastids).
机译：近年来植物血红素氧酶的研究由于其在各种环境压力期间的开发，防御和新陈代谢的几个作用，近年来考虑。在目前的研究中，评价血红素氧酶（HO）1的作用在减少重金属（CD和Ni）摄取和缓解CD和Ni毒性作用中的豇豆辐射成瓦的水殖种植幼苗。 PDM 54.幼苗在不同浓度的不同浓度下独立于10-100μm的氧化胁迫进行幼苗。在治疗96小时（第四天）后，收获应力植物以研究细胞稳态和排毒机制通过检查生长，应力参数（LPX，H 2 O 2含量）和非酶促和酶促参数（抗坏血酸过氧化物酶（APX），胍过氧化物酶（GPX）和过氧化氢酶（猫）），包括HO 1.在50μmcdcl2和60 mu m Niso4，HO 1活性在叶子中最高，分别为1.39和1.16倍。最大的HO 1活性从这些金属浓度（50μmCDCl2和60μmNO4）的降低的H 2 O 2含量反射，其与其他抗氧化酶的增加的活性相关（猫，APX）。因此，HO 1在一个组内工作，通过清除由时间依赖的研究确认的ROS来产生植物的生存机构的防御机械。因此，得出结论，V.Radiata的幼苗更耐受金属诱导的氧化应激，其中HO 1在其居民区（塑性）中局部化。

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