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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Jasmonic acid-induced NO activates MEK1/2 in regulating the metabolism of ascorbate and glutathione in maize leaves
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Jasmonic acid-induced NO activates MEK1/2 in regulating the metabolism of ascorbate and glutathione in maize leaves

机译:茉莉酸诱导没有激活MEK1 / 2在调节玉米叶中抗坏血酸和谷胱甘肽的代谢

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摘要

This study investigated the relationship between MEK1/2 and nitric oxide (NO) in jasmonic acid (JA)-regulated metabolism of ascorbate and glutathione in maize leaves. The results showed that JA increased the activities of APX, GR, MDHAR, DHAR, GalLDH, and gamma-ECS; the contents of AsA and GSH; and the production of NO. Above increases except for gamma-ECS activity and NO production were all suppressed by pre-treatments with MEK1/2 inhibitors PD98059 and U0126. Above increases were all suppressed by pre-treatments with nitric oxide synthase (NOS) inhibitor l-NAME and NO scavenger cPTIO. The results of western blot showed that JA enhanced the phosphorylation level of MEK1/2. Pre-treatments with l-NAME and cPTIO suppressed JA-induced phosphorylation level of MEK1/2. Our results suggested that JA-induced NO activated MEK1/2 by increasing the phosphorylation level, which, in turn, resulted in the upregulation of ascorbate and glutathione metabolism in maize leaves.
机译:本研究研究了茉莉酸(JA)的MeK1 / 2和一氧化氮(NO)之间的关系 - 在玉米叶中的抗坏血酸和谷胱甘肽的调节代谢。 结果表明,JA增加了APX,GR,MDHAR,DHAR,GALDH和GAMMA-EC的活动; ASA和GSH的内容; 和生产没有。 除了γ-ECS活性外,上述含量除外,没有生产全部抑制MEK1 / 2抑制剂PD98059和U0126。 通过用一氧化氮合酶(NOS)抑制剂L-NAME和NO清除剂CPTIO,通过预处理抑制了上述升高。 Western Blot的结果表明JA增强了MEK1 / 2的磷酸化水平。 预治疗L-名称和CPTIO抑制了JA诱导的MEK1 / 2的磷酸化水平。 我们的结果表明,通过增加磷酸化水平,JA-诱导活性MEK1 / 2,这反过来又导致玉米叶中抗坏血酸和谷胱甘肽代谢的上调。

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