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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Nitrogen nutrition modulates oxidative stress and metabolite production in Hypericum perforatum
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Nitrogen nutrition modulates oxidative stress and metabolite production in Hypericum perforatum

机译：氮营养调节氧化胁迫和血清孔的代谢产物

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摘要

Impact of various nitrate concentrations (14.12 mM, 3.53 mM, no nitrate) or ammonium presence (14.12 mM) on physiological and metabolic changes in Hypericum perforatum after 14 days of cultivation was monitored. Nitrate deficiency suppressed growth of shoots but stimulated root growth while ammonium suppressed root growth: concomitant changes of ascorbic acid and glutathione supported these growth changes, e.g., unaltered level in roots under nitrate deficiency but depleted in ammonium treatment. Soluble proteins and water content were more suppressed by nitrate deficiency but total ROS, nitric oxide formation, and antioxidative enzyme activities (APX and SOD) indicate higher sensitivity of plants to ammonium. Though both extreme treatments (NO3- deficiency or ammonium) stimulated accumulation of total soluble phenols and affected PAL activity (in comparison with full or 1/4x nitrate dose), major phenols (chlorogenic acid and three flavonoids) were elevated mainly by NO3- deficiency. At the level of specific metabolites, NO3- deficiency had stimulatory impact on pseudohypericin (but not hypericin) content while hyperforin decreased. Expression of earlier putative gene of hypericin biosynthesis (hyp-1) showed rather partial correlation with pseudohypericin amount. Data indicate that depletion of NO3- is useful to obtain Hypericum plants with higher amount of health-positive secondary metabolites.
机译：培养后，各种硝酸盐浓度（14.12mm，3.53mm，无硝酸盐）或铵存在（14.12mm）的影响受到培养后14天后金丝桃瘤的生理和代谢变化。硝酸盐缺乏抑制芽的生长，但刺激了根系生长，而铵抑制了根系生长：抗坏血酸和谷胱甘肽的伴随变化支持这些生长变化，例如硝酸盐缺乏下的根部的未妨碍水平，但在硝酸盐缺乏下含量。通过硝酸盐缺乏更抑制可溶性蛋白质和水含量，但总ROS，一氧化氮形成和抗氧化酶活性（APX和SOD）表明植物对铵的敏感性。虽然极端治疗（NO 3-缺乏或铵）刺激了总可溶性酚类和受影响的PAL活性（与全部或1 / 4x硝酸剂量相比），主要酚（绿原酸和三种类黄酮）主要由NO3缺乏升高。在特异性代谢物的水平下，NO3缺乏对伪华（但不是HINDICIN）含量的刺激性影响，而血清蛋白蛋白减少。先前推定基因的高素生物合成（HYP-1）的表达显示出与假上华量的相当部分相关性。数据表明NO 3的耗竭是有用的，可用于获得具有较高量的健康阳性二次代谢物的金刚素植物。

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