...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >The proteorhodopsins of the dinoflagellateOxyrrhis marina: ultrastructure and localization by immunofluorescence light microscopy and immunoelectron microscopy
【24h】

The proteorhodopsins of the dinoflagellateOxyrrhis marina: ultrastructure and localization by immunofluorescence light microscopy and immunoelectron microscopy

机译：DinoflagellateOxyrrhis Marina的蛋白质醇：通过免疫荧光光学显微镜和免疫电解显微镜的超微结构和定位

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

At least 7 proteorhodopsin sequences ofOxyrrhis marinawere recently proven in bands obtained by sucrose density gradient centrifugation, and MS analyses revealed that the bands consisted almost of pure, native proteorhodopsins (Rhiel et al.2020). The proteorhodopsin fractions, i.e., bands B2, B3, and B4 were subjected to transmission electron microscopy. Negative staining revealed that band B2 consisted most likely of monomeric/oligomeric proteorhodopsins with particle dimensions of about 6 nm. Negative staining, freeze-fracture, and cryo-transmission electron microscopy revealed that bands B3 and B4 consisted of vesicular, sheet-like, and cup-shaped structures which all seemed to be composed of protein. Frequently, ring-like protein aggregates were registered at higher magnifications. They measured about 4 nm in diameter with a tiny hole of 1.5 nm in the middle. The bands B2, B3, and B4 were pooled and used to raise an antiserum. Immunoelectron microscopy resulted in intense labeling of the isolated structures. Immunofluorescence light microscopy of formaldehyde-fixedOxyrrhiscells resulted in intense labeling of the cell periphery. Some cell internal structures became labeled, too. Immunoelectron microscopy of freeze-fractured cells revealed that most likely the membranes of the amphiesmal vesicles were labeled at the cell periphery, while the cell internal label seemed to originate from the food vacuoles.
机译：最近在蔗糖密度梯度离心和MS分析中获得的40SOXYRRHIS MARINAWERE of oxOxyrrhis MarinaWere序列，并且MS分析表明，带子几乎组成了纯的天然蛋白质酚（RHIEL等，Al.2020）。对蛋白质孢子馏分，即带B2，B3和B4进行透射电子显微镜。负染色显示，带B2的颗粒尺寸为约6nm的单体/寡聚蛋白孢囊最有可能。负染色，冷冻骨折和冷冻透射电子显微镜显示，带B3和B4由囊状，片状和杯形结构组成，尤其如此似乎由蛋白质组成。通常，环状蛋白质聚集体在更高的放大率下注册。它们的直径测量约4nm，中间有1.5nm的微小孔。合并带B2，B3和B4并用于提高抗血清。免疫电解显微镜导致隔离结构的强烈标记。甲醛 - 固定毒液的免疫荧光光学显微镜显微镜导致细胞周边的强烈标记。一些细胞内部结构也被标记为标记。冷冻裂缝细胞的免疫电解显微镜显示，最有可能在细胞周边标记Amphiesmal囊泡的膜，而细胞内标似乎源自食物真空。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号