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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Amycolatopsis alkalitolerans sp. nov., isolated from Gastrodia elata Blume
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Amycolatopsis alkalitolerans sp. nov., isolated from Gastrodia elata Blume

机译：amycolatopsis碱碱sp。 11月。，从胃杉elata布卢姆孤立

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摘要

A Gram-staining positive and nonmotile strain designated SYSUP0005T was isolated from tubers of Gastrodia elata Blume. The 16S rRNA gene sequence result showed that strain SYSUP0005T shared highest sequence similarity with the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%). Growth occurs at 14–37?°C (optimum temperature, 28?°C), at pH 6–9 (optimum, pH 8) and in the presence of up to 6% (w/v) NaCl. Strain SYSUP0005T had meso-diaminopimelic acid in its peptidoglycan. The whole cell sugars were galactose, ribose, and xylose. The predominant menaquinone was MK-9(H4) and minor menaquinones were MK-9(H2) and MK-9(H8). The polar lipids were diphosphatidylglycerol (DPG); phosphatidylmonomethylethanolamine (PME), phosphatidylethanolamine (PE), phosphatidylinositol (PI), unidentified glycolipid (GL), and unidentified phospholipid (PL). The genomic DNA G?+?C content was 69.6?mol%. The major fatty acids were iso-C16:0, anteiso-C17:0, C16:0, iso-C14:0, C17:1 ω6c, C17:0, and Summed Feature 3 (C16:1 ω7c/C16:1 ω6c). On the basis of the phenotypic, phylogenetic, chemotaxonomic characters, and genomic comparison, SYSUP0005T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis alkalitolerans sp. nov. is proposed. The type strain is SYSUP0005T (=KCTC 49024T?=?CGMCC4.7463T).
机译：从胃塔植物乌雷塔布卢姆的块茎分离出一个克染色的阳性和非墨水菌株指定的Sysup0005t。 16S rRNA基因序列结果表明，菌株SYSUP0005T与杏仁糖尿病菌株（95.7％），amycolaTopsis Taiwansis（95.4％），amycolatopsis pigmentata（95.4％），amycolatopsis ruanii（95.1％）和amycolatopsis herveola（95.1％）和杏仁糖（94.8％）。生长发生在14-37℃（最佳温度，28Ω℃），pH 6-9（最佳，pH8）和高达6％（w / v）NaCl存在下。菌株Sysup0005T在其肽聚糖中具有中学二氨基纤维酸。整个细胞糖是半乳糖，核糖和木糖。主要的menaquinone是MK-9（H4）和轻微的母牛是MK-9（H2）和MK-9（H8）。极性脂质是二磷脂酰甘油（DPG）;磷脂酰甲基乙醇胺（PME），磷脂酰乙醇胺（PE），磷脂酰肌醇（PI），未识别的糖脂（GL）和未识别的磷脂（PL）。基因组DNA g？+αc含量为69.6Ω·摩尔％。主要脂肪酸是ISO-C16：0，ANTEISO-C17：0，C16：0，ISO-C14：0，C17：1Ω6C，C17：0和求和特征3（C16：1Ω7c/ c16：1Ω6c ）。基于表型，系统发育，趋化性的特征和基因组比较，Sysup0005t代表了amycolaTopsis属的新种类，其中名称是amycolaTopsis碱碱SP。 11月。提出。类型应变为sysup0005t（= kctc 49024t？=？cgmcc4.7463t）。

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