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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Paper-based luminescence bioassay method embedding a sequence of enzymatic reactions to detect sulfonamide groups
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Paper-based luminescence bioassay method embedding a sequence of enzymatic reactions to detect sulfonamide groups

机译：基于纸的发光生物测定方法嵌入一系列酶促反应以检测磺酰胺基团

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摘要

Sulfonamide residue in foodstuffs and the environment is a serious global concern for their contribution to the occurrence of antibiotic-resistant bacteria, especially in developing countries. Here, we describe a novel, simple, and low-cost bioassay for sulfonamides, which has high potential versatility for use in low-resource settings. The bioassay method is based on a purpose-built luminescent assay reaction that detects sulfonamide groups. The luminescent assay reaction comprises dihydropteroate synthase, a target enzyme of sulfonamides, and luminescent pyrophosphate detection reagent, which triggers a sequence of biomolecular reactions that convert sulfonamides to emit luminescence. The novel assay detected at least six different sulfonamides with an estimated limit of detection of 25 ng ml(-1) in a solution-phase assay using a microplate reader. More importantly, the luminescent assay reaction functioned even after spotting and freeze-drying on a wax pattern-printed paper platform. The paper-embedded luminescent assay reaction showed response signals to sulfadiazine within 30 min at a limit of detection similar to that of the solution-phase assay using a microplate reader. The signal could be recorded using a digital camera in the dark and required no other laboratory infrastructure, freeing the assay from the constraints of a well-fitted laboratory.
机译：食品中的磺胺酰胺残留物和环境是对它们对抗生素抗性细菌发生贡献的严重全球关注，特别是在发展中国家。在这里，我们描述了一种用于磺胺酰胺的新颖，简单，低成本的生物测定，其具有高潜在的多功能性，用于低资源设置。生物测定方法基于检测磺酰胺基团的目的制造的发光测定反应。发光测定反应包括二氢酸盐合酶，磺酰胺的靶酶，以及发光焦磷酸磷酸磷酸磷酸磷酸磷酸磷酸磷酸磷酸盐检测试剂，其触发了转化磺酰胺的生物分子反应序列以发射发光。使用微孔板读卡器检测到具有至少六种不同的磺酰胺，其估计在溶液相测定中检测估计的含量限制。更重要的是，即使在蜡图案印刷纸平台上发现和冷冻干燥后，发光测定的反应也能发挥作用。纸嵌入的发光测定反应显示在使用微孔板读取器的检测限时在30分钟内向磺胺腈显示响应信号。可以在黑暗中使用数码相机记录信号，并且无需其他实验室基础设施，从拟合实验室的限制中释放测定。

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    Ujiie Kazuki; Matsuura Hideyuki; Tran Thi My Duyen; Izutsu Koki; Nitta Ayaka; Harada Kazuo; Hirata Kazumasa;

  • 作者单位

    Osaka Univ Grad Sch Pharmaceut Sci 1-6 Yamadaoka Suita Osaka 5650871 Japan;

    Osaka Univ Grad Sch Pharmaceut Sci 1-6 Yamadaoka Suita Osaka 5650871 Japan;

    Osaka Univ Grad Sch Pharmaceut Sci 1-6 Yamadaoka Suita Osaka 5650871 Japan;

    Osaka Univ Grad Sch Pharmaceut Sci 1-6 Yamadaoka Suita Osaka 5650871 Japan;

    Osaka Univ Grad Sch Pharmaceut Sci 1-6 Yamadaoka Suita Osaka 5650871 Japan;

    Osaka Univ Grad Sch Pharmaceut Sci 1-6 Yamadaoka Suita Osaka 5650871 Japan;

    Osaka Univ Grad Sch Pharmaceut Sci 1-6 Yamadaoka Suita Osaka 5650871 Japan;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 药学;
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