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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Genetic analysis of Helicobacter pylori clinical isolates suggests resistance to metronidazole can occur without the loss of functional rdxA.
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Genetic analysis of Helicobacter pylori clinical isolates suggests resistance to metronidazole can occur without the loss of functional rdxA.

机译：幽门螺杆菌临床分离株的遗传分析表明，在不损失功能RDXA的情况下会发生对甲硝唑的抗性。

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摘要

Resistance to metronidazole (MTZ) in Helicobacter pylori is associated with mutations in rdxA, encoding an oxygen-insensitive NADPH nitroreductase, and mutations in frxA, encoding a NAD(P)H-flavin oxidoreductase. Despite this association, the strict correlation of MTZ resistance with mutations in rdxA or frxA is still controversial. In this study, rdxA allelic replacement was used to distinguish resistance-associated nucleotide mutations from the natural genetic diversity of H. pylori. Replacement with truncated rdxA resulted in MTZ resistance, whereas replacement with missense-mutated rdxA from resistant clinical isolates failed to yield MTZ resistance. Thus, although truncation of rdxA confers MTZ resistance in G27 H. pylori, MTZ resistance found in other clinical isolates is not due to the identified amino-acid substitutions. Three of our MTZ-resistant clinical isolates expressed functional rdxA and two of these also encoded full-length frxA. Therefore, MTZ resistance can arise in H. pylori possessing functional rdxA, suggesting that other factors are involved in MTZ resistance.
机译：对幽门螺杆菌的抗性甲硝唑（MTZ）与RDXA中的突变相关，编码氧不敏感NADPH硝化酶，以及FRXA中的突变，编码NAD（P）H-黄蛋白氧化还原酶。尽管这种协会，但MTZ抗性与RDXA或FRXA突变的严格相关性仍然存在争议。在该研究中，RDXA等位基因替代物用于区分抗性相关的核苷酸突变与幽门螺杆菌的自然遗传多样性。截断的RDXA替代导致MTZ抗性，而用抗性突变的RDXA替代抗性临床分离株未能产生MTZ抗性。因此，尽管在G27 H中的RDXA赋予MTZ抗性的致盲肠抗性，但在其他临床分离株中发现的MTZ抗性不是由于所鉴定的氨基酸取代。我们的三个MTZ抗性临床分离株表达了功能性RDXA，其中两种也编码了全长FRXA。因此，MTZ抗性可以在具有功能性RDXA的幽门中出现，表明其他因素涉及MTZ抗性。

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