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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Substrate specificity of radical S-adenosyl-L-methionine dehydratase AprD4 and its partner reductase AprD3 in the C3 '-deoxygenation of aminoglycoside antibiotics
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Substrate specificity of radical S-adenosyl-L-methionine dehydratase AprD4 and its partner reductase AprD3 in the C3 '-deoxygenation of aminoglycoside antibiotics

机译：基氨酸S-腺苷-1-甲硫氨酸脱水酶APRD4及其合作蛋白酶还原酶APRD3的底物特异性在氨基糖苷类抗生素的C3'-羟基中的APRD3

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摘要

A radical S-adenosyl-L-methionine dehydratase AprD4 and an NADPH-dependent reductase AprD3 are responsible for the C3'-deoxygenation of pseudodisaccharide paromamine in the biosynthesis of apramycin. These enzymes are involved in the construction of the characteristic structural motif that is not modified by 3'-phosphotransferase in aminoglycoside-resistant bacterial strains. AprD4 catalyzes the C3'-dehydration of paromamine via a radical-mediated reaction mechanism to give 4'-oxolividamine, which is then reduced by AprD3 with NADPH to afford lividamine. In the present study, the substrate specificity of this unique combination of enzymes has been investigated. AprD4 was found to recognize paromamine, neamine, kanamycin C, and kanamycin B to afford 5'-deoxyadenosine as one of products during the C3'-dehydration of aminoglycosides, but not 2'-N-acetylparomamine and paromomycin. Only paromamine and kanamycin C were converted to the corresponding C3'-deoxygenated compounds by AprD4 and AprD3. AprD3 recognizes the 4'-oxolividamine moiety, including the pseudotrisaccharide kanamycin C, and seems to reject the amino group at C6' of neamine and kanamycin B. Chirally deuterium-labeled NADPH was used to identify that that AprD3 transfers the pro-S hydrogen atom of NADPH when reducing 4'-oxolividamine to give lividamine.
机译：一种基团S-腺苷-1-甲硫氨酸脱水酶APRD4和NADPH依赖性还原酶APRD3是在尿嘧啶的生物合成中的假二糖甲氨基的C3'-脱氧。这些酶参与结构的特征结构基质​​，其在氨基苷抗性细菌菌株中未被3'-磷光转移酶修饰的特征结构基质​​。 APRD4通过自由基介导的反应机制催化托胺的C3'脱水，得到4'- oxolidamine，然后通过NADPH提供4'- oxolidamine，以提供Lividamine。在本研究中，研究了这种独特的酶组合的底物特异性。发现APRD4识别帕氨胺，奈良，卡那霉素C和Kanamycin B，得到5'-脱氧腺苷作为在氨基糖苷的C3-脱水过程中的一种产品之一，但不是2'-N-乙酰基甲胺和偏霉素。仅在4月4日和APRD3转化为相应的C3'-脱氧化合物的副胺和卡那霉素C. APRD3认识到4'- oxolidamine部分，包括假性三糖类霉素C，似乎拒绝了Neminine和Kanamycin B的C6'的氨基。手术人氘标记的NADPH用于鉴定APRD3转移PRO-S氢原子在减少4'- oxolidamine时NADPH给予杨柳。

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