...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of Antibiotics: An International Journal >Molecular beacons to identify gifted microbes for genome mining
【24h】

Molecular beacons to identify gifted microbes for genome mining

机译：分子信标以确定基因组采矿的有天赋的微生物

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Microbial genome mining is a promising technology that is revitalizing natural product discovery. It is now well documented that many bacteria with large genomes, particularly actinomycetes, encode many more secondary metabolites (SMs) than was previously known from their expressed secondary metabolomes. There are effective bioinformatics tools for counting the numbers and nature of SMs, and determining the total coding capacity from finished microbial genomes. However, these methods do not translate well to draft genomes, particularly for large SM gene clusters that contain nonribosomal peptide synthetase (NRPS) or type I polyketide synthase (PKS- I) mega-genes which are prone to fragmentation and misassembly. Small molecular beacons are required to assess the numbers and variety of NRPS, PKS- I and mixed NRPS/PKS-I pathways. In this report, I show that concatenated peptidyl carrier protein-thioesterase di-domains and acyl carrier protein-thioesterase di-domains can be used as multi-probes to survey finished or draft genomes to estimate the numbers of NRPS, PKS- I and mixed NRPS/PKS-I gene clusters to identify gifted actinomycetes.
机译：微生物基因组采矿是一种有希望的技术，可振兴自然产品发现。现在有充分的记录说，许多具有大型基因组，特别是放线菌的细菌，编码了比以前从其表达的次级代谢物中已知的更多次生代谢物（SMS）。有有效的生物信息学工具，用于计算SMS的数量和性质，并确定完成微生物基因组的总编码能力。然而，这些方法不会很好地转化为草拟基因组，特别是对于含有非纤维素肽合成酶（NRP）或I型聚酮化合物合酶（PKS-I）的大型SM基因簇，其易于破碎和遗传物质。需要小分子信标评估NRPS，PKS-I和混合NRPS / PKS-I路径的数量和各种。在本报告中，我表明串联肽基载体蛋白 - 硫酯酶二畴和酰基载体蛋白 - 硫酯酶二域可以用作调查完成或草稿基因组的多探针，以估计NRP，PKS- I和混合的数量NRPS / PKS-I基因集群识别有天赋的放线菌。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号