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首页> 外文期刊>The Journal of Antibiotics: An International Journal >NovQ is a prenyltransferase capable of catalyzing the addition of a dimethylallyl group to both phenylpropanoids and flavonoids.
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NovQ is a prenyltransferase capable of catalyzing the addition of a dimethylallyl group to both phenylpropanoids and flavonoids.

机译：NovQ是戊基转移酶，能够催化将二甲基丙烯基的添加到苯基丙醇和黄酮类化合物中。

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摘要

NovQ is a member of a recently identified CloQ/NphB class of prenyltransferases. Although NphB has been well characterized as a prenyltransferase with flexibility against aromatic substrates, few studies have been carried out on characterization of NovQ. Hence, in this study, we investigate the kinetics, substrate specificity and regiospecificity of NovQ. The corresponding novQ gene was cloned from Streptomyces niveus, which produces an aminocoumarin antibiotic, novobiocin. Recombinant NovQ was overexpressed in Escherichia coli and purified to homogeneity. The purified enzyme was a soluble monomeric 40-kDa protein that catalyzed the transfer of a dimethylallyl group to 4-hydroxyphenylpyruvate (4-HPP) independently of divalent cations to yield 3-dimethylallyl-4-HPP, an intermediate of novobiocin. Steady-state kinetic constants for NovQ with the two substrates, 4-HPP and dimethylallyl diphosphate, were also calculated. In addition to the prenylation of 4-HPP, NovQ catalyzed carbon-carbon-based and carbon-oxygen-based prenylations of a diverse collection of phenylpropanoids, flavonoids and dihydroxynaphthalenes. Despite its catalytic promiscuity, the NovQ-catalyzed prenylation occurred in a regiospecific manner. NovQ is the first reported prenyltransferase capable of catalyzing the transfer of a dimethylallyl group to both phenylpropanoids, such as p-coumaric acid and caffeic acid, and the B-ring of flavonoids. This study shows that NovQ can serve as a useful biocatalyst for the synthesis of prenylated phenylpropanoids and prenylated flavonoids.
机译：Novq是最近鉴定的Cloq / NPHB类植物转移酶的成员。虽然NHB已经很好地表征为戊基转移酶，但是对芳族底物的灵活性进行了灵活性，但已经对NOVQ的表征进行了很少的研究。因此，在这项研究中，我们研究了Novq的动力学，底物特异性和细胞质。将相应的NovQ基因从Streptomyces Niveus克隆，其产生氨基脲抗生素，Novobiocin。重组Novq在大肠杆菌中过表达，纯化为均匀性。纯化的酶是一种可溶性单体40-KDA蛋白，其催化二甲基丙烯基团至4-羟基苯吡啶磺酸盐（4-HPP）的转移，独立于二价阳离子，得到3-二甲基all-4- HPP，Novobiocin的中间体。还计算了NoVQ的稳态动力学常数，具有两个基材，4-HPP和二甲基二磷酸二磷酸。除了4-HPP，NovQ催化的碳 - 碳基和碳 - 氧基戊烷的外，还包括多种苯丙醇，黄酮类化合物和二羟基萘。尽管其催化滥交，但NovQ催化的戊烯化以再生形式发生。 Novq是第一个能够催化二甲基丙烷基团的转移至苯基丙醇，例如对香豆酸和咖啡酸的转移，以及黄酮类化合物的戊基转移酶。本研究表明，NovQ可以作为合成戊基苯丙醇和戊烷化的类黄酮的有用生物催化剂。

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