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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Monooxygenation of rifampicin catalyzed by the rox gene product of Nocardia farcinica: structure elucidation, gene identification and role in drug resistance.
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Monooxygenation of rifampicin catalyzed by the rox gene product of Nocardia farcinica: structure elucidation, gene identification and role in drug resistance.

机译：Nocardia Farcinica的ROX基因产物催化的利福平产品的单氧化：结构阐明，基因鉴定和耐药性的作用。

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摘要

We demonstrated that the rox gene of Nocardia farcinica encodes a rifampicin monooxygenase capable of converting rifampicin to a new compound 2'-N-hydroxy-4-oxo-rifampicin with a markedly lowered antibiotic activity. The deletion mutation (Deltarox) of the rox gene gave no significant influence to the rifampicin resistance of N. farcinica. However, transformation with a plasmid containing an overexpressing the rox gene markedly raised the rifampicin resistance in the strain with the deletion mutation of the rpoB2 gene as the principal rifampicin resistance determinant. On the other hand, rifampicin was decolorized by the wild-type strain, whereas it remained intact when incubated with the Deltarox strain. Based on these results, it will be conclusive that the rox gene is capable of initiating rifampicin degradation with a new metabolite formation at the first step and having a role as the secondary rifampicin resistance factor in N. farcinica.
机译：我们证明Nocardia Farcinica的Rox基因编码了能够将利福平的单氧基酶转化为新的化合物2'-N-羟基-4-氧代 - 利福平，并且具有显着降低的抗生素活性。 ROX基因的缺失突变（Deltarox）对N.Farcinica的利福平抗性没有显着影响。 然而，用含有过表达的质粒转化ROX基因明显提高了菌株中的利福平素抗性，随着RPOB2基因的缺失突变为主要的利福平抗性决定簇。 另一方面，利福平通过野生型菌株脱色，而当与Deltarox菌株温育时，它保持完整。 基于这些结果，它将得出决定性的是，ROX基因能够在第一步并在第一步中引发利福平的抗降解，并在N.Arcinica中具有次级利福平抗性因子。

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