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首页> 外文期刊>The Journal of Antibiotics: An International Journal >Isolation and characterization of a new cyclic lipopeptide orfamide H from Pseudomonas protegens CHA0
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Isolation and characterization of a new cyclic lipopeptide orfamide H from Pseudomonas protegens CHA0

机译：来自假单胞菌氏族CHA0的新循环脂肽矿石H的分离与表征

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摘要

A new cyclic lipopeptide (CLP) orfamide H (1) was purified and identified from the cultural broth of the bacterial strain Pseudomonas protegens CHA0. The crude extract of the strain CHA0 was obtained by an acid-aided precipitation process, then the compound 1 was purified by reversed-phase high-performance liquid chromatography (RP-HPLC). Subsequently, the chemical structure of orfamide H was determined by 1D and 2D nuclear magnetic resonance (NMR) and mass spectrometry (MS). Further biological assays indicate that the new CLP orfamide H shows the activity on inhibiting the appressoria formation of the fungus Magnaporthe oryzae, the causal agent of the blast disease in rice. Taken all together, these results indicated that the new CLP orfamide H has the capacity to be developed as an agrichemical to control blast disease in rice.
机译：纯化新的环状脂肽（CLP）Orfamide H（1），并从细菌菌株Pseudomonas氏族Cha0的培养液中鉴定。 菌株CHA0的粗提物通过酸辅助沉淀法得到，然后通过反相高效液相色谱（RP-HPLC）纯化化合物1。 随后，通过1D和2D核磁共振（NMR）和质谱法（MS）测定Orfamide H的化学结构。 进一步的生物学测定表明，新的CLP Orfamide H显示了抑制真菌Magnaporthe Oryzae的孕产量，水稻中爆炸疾病的因果剂的活性。 所有这些结果都表明，新的CLP Orfamide H具有作为对水稻爆炸疾病进行制备的能力。

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  • 作者

    Ma Zongwang; Zhang Songya; Liang Junyu; Sun Kun; Hu Jiangchun;

  • 作者单位

    Northwest Normal Univ Coll Life Sci 967 East Anning Rd Lanzhou 730070 Peoples R China;

    Chinese Acad Sci Inst Synthet Biol Shenzhen Inst Adv Technol 1068 Xueyuan Rd Shenzhen 518055;

    Northwest Normal Univ Coll Life Sci 967 East Anning Rd Lanzhou 730070 Peoples R China;

    Northwest Normal Univ Coll Life Sci 967 East Anning Rd Lanzhou 730070 Peoples R China;

    Chinese Acad Sci Inst Appl Ecol 72 Wenhua Rd Shenyang 110016 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 药学;
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