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首页> 外文期刊>The Journal of craniofacial surgery >Bone-Conditioned Medium Obtained From Calvaria, Mandible, and Tibia Cause an Equivalent TGF-1 Response In Vitro
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Bone-Conditioned Medium Obtained From Calvaria, Mandible, and Tibia Cause an Equivalent TGF-1 Response In Vitro

机译：从Calvaria，下颌骨和胫骨获得的骨状调节培养基导致体外等效的TGF-1反应

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摘要

Bones with different embryological origin and mode of ossification are supposed to vary in their capacity for supporting graft consolidation. The aim of the current pilot study was to assess the TGF-1 activity of bone chips obtained from distinct anatomic locations. Conditioned medium was prepared from bone chips harvested from pig calvaria, mandible, and tibia. Human oral fibroblasts were exposed to bone-conditioned medium (BCM) followed by reverse transcriptase polymerase chain reaction of the TGF-1 target genes. Also an immunoassay for interleukin 11 (IL-11) and TGF-1 was performed. The impact of BCM on alkaline phosphatase activity was determined with murine MC3T3-E1 osteogenic cells. The authors report here that BCM contains TGF-1 in the ng/mL range. Bone chips prepared from pig calvaria, mandible, and tibia femur had a similar capacity for increasing the expression of the TGF-1 target genes IL-11, NOX4, and PRG4. Correspondingly, immunoassays revealed similar production of IL-11 by human oral fibroblasts. Furthermore, conditioned medium obtained from the 3 bones decreased alkaline phosphatase activity in MC3T3-E1 osteogenic cells. These preliminary data demonstrate that particulated bone grafts, regardless of embryological origin, mode of ossification and morphology, release a similar TGF-1 activity.
机译：具有不同胚胎的骨骼和骨化模式的骨骼应该以其支持移植巩固的能力而变化。目前的试验研究的目的是评估从不同解剖位置获得的骨屑的TGF-1活性。条件培养基由从猪Calvaria，下颌骨和胫骨收获的骨屑制备。将人口腔成纤维细胞暴露于骨状调节培养基（BCM），然后逆转录酶聚合酶链反应的TGF-1靶基因。还进行白细胞介素11（IL-11）和TGF-1的免疫测定。用鼠MC3T3-E1骨质发生细胞测定BCM对碱性磷酸酶活性的影响。作者在此报告，BCM在NG / ML范围内包含TGF-1。由猪Calvaria，下颌骨和胫骨股骨制备的骨芯片具有类似的能力，以增加TGF-1靶基因IL-11，NOX4和PRG4的表达。相应地，免疫测定显示了人口腔成纤维细胞的类似IL-11的产生。此外，从3个骨中获得的条件培养基在MC3T3-E1骨质发生细胞中降低了碱性磷酸酶活性。这些初步数据表明，颗粒状骨移植物，无论胚胎源，骨化的模式和形态，释放出类似的TGF-1活性。

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