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首页> 外文期刊>The Journal of dermatology >Differential expression analysis of miRNA in peripheral blood mononuclear cells of patients with non-segmental vitiligo
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Differential expression analysis of miRNA in peripheral blood mononuclear cells of patients with non-segmental vitiligo

机译：非节段性白癜风患者外周血单核细胞miRNA的差异表达分析

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Vitiligo is a common depigmentary skin disease that may follow a pattern of multifactorial inheritance. The essential factors of its immunopathogenesis is thought to be the selective destruction of melanocytes. As a new class of microregulators of gene expression, miRNA have been reported to play vital roles in autoimmune diseases, metabolic diseases and cancer. This study sought to characterize the different miRNA expression pattern in the peripheral blood mononuclear cells (PBMC) of patients with non-segmental vitiligo (NSV) and healthy individuals and to examine their direct responses to thymosin 1 (T1) treatment. The miRNA expression profile in the PBMC of patients with NSV was analyzed using Exiqon's miRCURY LNA microRNA Array. The differentially expressed miRNA were validated by real-time quantitative polymerase chain reaction. We found that the expression levels of miR-224-3p and miR-4712-3p were upregulated, and miR-3940-5p was downregulated in the PBMC. The common clinical immune modulator T1 changed the miRNA expression profile. Our analysis showed that differentially expressed miRNA were associated with the mechanism of immune imbalance of vitiligo and that T1 could play an important role in changing the expression of these miRNA in the PBMC of patients with NSV. This study provided further evidence that miRNA may serve as novel drug targets for vitiligo therapeutic evaluation.
机译：白癜风是一种常见的甲状腺皮肤病，可能遵循多因素遗产的模式。其免疫病变的基本因素被认为是黑素细胞的选择性破坏。作为基因表达的一类新的微型微观调用剂，据报道，miRNA在自身免疫疾病，代谢疾病和癌症中起重要作用。该研究寻求在非节段性白癜风（NSV）和健康个体患者的外周血单核细胞（PBMC）中的不同miRNA表达模式，并检查它们对胸腺嘧啶1（T1）处理的直接反应。使用Exiqon的Mircury LNA MicroRNA阵列分析了NSV患者PBMC中的miRNA表达谱。通过实时定量聚合酶链反应验证差异表达的miRNA。我们发现MiR-224-3P和MIR-4712-3P的表达水平被上调，并且在PBMC中下调miR-3940-5p。常见的临床免疫调节剂T1改变了miRNA表达谱。我们的分析表明，差异表达的miRNA与白癜风的免疫失调机制有关，并且T1可以在改变NSV患者PBMC中的这种miRNA表达方面发挥重要作用。本研究提供了进一步证明MiRNA可以作为白癜风治疗评价的新药靶标。

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