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首页> 外文期刊>The Journal of Horticultural Science & Biotechnology >Male flower induction significantly affects androgenesis in cucumber (Cucumis sativus L.)
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Male flower induction significantly affects androgenesis in cucumber (Cucumis sativus L.)

机译：雄性花诱导显着影响黄瓜（Cucumis Sativus L.）的雄激素

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摘要

Male flower induction and androgenesis in three genotypes of cucumber (Cucumis sativus), two Beth alpha F1 hybrids (BT1 and BT2, gynoecious) and Dastgerdi (DTG, monoecious), were evaluated. Induction of male flowers in gynoecious cucumber cultivars was assessed by single and combinatorial spraying of AgNO3 (3 mM), GA(3) (1.5 mM) and CoCl2 (3 mM) after the first true leaf emergence. After 6 weeks from the beginning of treatments, the average numbers of male flowers induced by spraying AgNO3, GA(3), and CoCl2 were 42, 14.5, and 0, respectively. Androgenesis was performed in two gynoecious cultivars, in which the donor plants were treated with AgNO3 (3 mM) or GA(3) (1.5 mM) and the DTG cultivar without treatment. Among the three cultivars, the DTG showed the highest percentage of embryogenic calli formation (62.2%) and number of embryos per anther (1.81). In the gynoecious cultivars, the percentage of embryogenic calli formation (27.1%) and number of embryos per anther (0.23) in the anthers of GA(3) treated plants were significantly higher than those treated by AgNO3. Cytological and SSR marker analysis of regenerants showed that 16 plants were spontaneous doubled haploid, 2 plants were diploid and one plant was tetraploid.
机译：评估了雄性花诱导和AndRogesis的黄瓜（Cucumis Sativus），两种BethαF1杂交种（BT1和BT1和BT2，淫乱）和Dastgerdi（DTG，单生成）中的三种基因分型。在第一次真实叶片出现后，通过单一和组合喷涂，通过单一和组合喷涂来评估雌性黄瓜品种中的雄性花卉的诱导。在处理开始6周后，通过喷涂AgNO 3，Ga（3）和COCl2引起的雄性花的平均数分别为42,14.5和0。在两个营养品种中进行雄激素，其中供体植物用AgNO 3（3mM）或Ga（3）（1.5mm）和DTG品种而无需处理。在三种品种中，DTG显示出最高百分比的胚性愈伤组织形成（62.2％）和每个花药（1.81）的胚胎数。在纯品种中，Ga（3）处理植物的花药中胚状愈伤组织形成（27.1％）和每种花药（0.23）的胚胎数明显高于AgNO3处理的植物中的每种花药。再生细胞学和SSR标记分析表明，16株植物自发加倍单倍体，2株植物是二倍体，一株植物是四倍体。

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