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首页> 外文期刊>The ISME journal emultidisciplinary journal of microbial ecology >Long-term succession in a coal seam microbiome during in situ biostimulation of coalbed-methane generation
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Long-term succession in a coal seam microbiome during in situ biostimulation of coalbed-methane generation

机译：煤层气煤层生物刺激期间煤层微生物梭中长期连续

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Despite the significance of biogenic methane generation in coal beds, there has never been a systematic long-term evaluation of the ecological response to biostimulation for enhanced methanogenesis in situ. Biostimulation tests in a gas-free coal seam were analysed over 1.5 years encompassing methane production, cell abundance, planktonic and surface associated community composition and chemical parameters of the coal formation water. Evidence is presented that sulfate reducing bacteria are energy limited whilst methanogenic archaea are nutrient limited. Methane production was highest in a nutrient amended well after an oxic preincubation phase to enhance coal biofragmentation (calcium peroxide amendment). Compound-specific isotope analyses indicated the predominance of acetoclastic methanogenesis. Acetoclastic methanogenic archaea of the Methanosaeta and Methanosarcina genera increased with methane concentration. Acetate was the main precursor for methanogenesis, however more acetate was consumed than methane produced in an acetate amended well. DNA stable isotope probing showed incorporation of 13C-labelled acetate into methanogenic archaea, Geobacter species and sulfate reducing bacteria. Community characterisation of coal surfaces confirmed that methanogenic archaea make up a substantial proportion of coal associated biofilm communities. Ultimately, methane production from a gas-free subbituminous coal seam was stimulated despite high concentrations of sulfate and sulfate-reducing bacteria in the coal formation water. These findings provide a new conceptual framework for understanding the coal reservoir biosphere.
机译：尽管生物甲烷生成在煤层中的重要性，但从未有过系统的长期评价对生物抑制的生态反应，以原位增强的甲烷化。在煤层生产，细胞丰度，浮游和表面相关群落组合物和煤炭形成水的化学参数，分析了无煤层中的生物刺激试验。提出了硫酸盐还原细菌是能量有限的，而甲状腺原核是营养有限的。在氧化前孵化相后，培养物的培养基中的培养基中的甲烷产量最高，以增强煤生物腐烂（过氧化物修正）。复合特异性同位素分析表明乙酰型甲烷化的主要素。甲烷酸甲基甲基和甲基喹啉甲酸的乙酰型甲状腺原酸型随甲烷浓度而增加。乙酸盐是甲烷发生的主要前体，然而，在乙酸盐中生产的甲烷消耗更多的乙酸盐。 DNA稳定同位素探测显示将13℃标记的乙酸盐掺入甲状腺原子，地形分类物种和硫酸盐还原细菌中。煤表面的社区表征证实，甲状腺原核构成了大量煤相关生物膜社区。最终，尽管煤炭形成水中高浓度的硫酸盐和硫酸盐还原细菌，因此刺激了来自无气体脱壳煤层的甲烷生产。这些调查结果为了解煤储层生物圈提供了一种新的概念框架。

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