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首页> 外文期刊>The ISME journal emultidisciplinary journal of microbial ecology >A novel bacterial thiosulfate oxidation pathway provides a new clue about the formation of zero-valent sulfur in deep sea
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A novel bacterial thiosulfate oxidation pathway provides a new clue about the formation of zero-valent sulfur in deep sea

机译：一种新的细菌硫代硫酸盐氧化途径为深海中零价硫的形成提供了新的线索

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摘要

Zero-valent sulfur (ZVS) has been shown to be a major sulfur intermediate in the deep-sea cold seep of the South China Sea based on our previous work, however, the microbial contribution to the formation of ZVS in cold seep has remained unclear. Here, we describe a novel thiosulfate oxidation pathway discovered in the deep-sea cold seep bacteriumErythrobacter flavus21-3, which provides a new clue about the formation of ZVS. Electronic microscopy, energy-dispersive, and Raman spectra were used to confirm thatE. flavus21-3 effectively converts thiosulfate to ZVS. We next used a combined proteomic and genetic method to identify thiosulfate dehydrogenase (TsdA) and thiosulfohydrolase (SoxB) playing key roles in the conversion of thiosulfate to ZVS. Stoichiometric results of different sulfur intermediates further clarify the function of TsdA in converting thiosulfate to tetrathionate (-O3S-S-S-SO3-), SoxB in liberating sulfone from tetrathionate to form ZVS and sulfur dioxygenases (SdoA/SdoB) in oxidizing ZVS to sulfite under some conditions. Notably, homologs of TsdA, SoxB, and SdoA/SdoB widely exist across the bacteria including inErythrobacterspecies derived from different environments. This strongly indicates that this novel thiosulfate oxidation pathway might be frequently used by microbes and plays an important role in the biogeochemical sulfur cycle in nature.
机译：零价硫磺（ZVS）已被证明是南海深海冷渗透的主要中间体，但基于我们以前的工作，然而，对冷渗透ZVS形成的微生物贡献仍不清楚。在此，我们描述了在深海冷渗菌细菌Flavus21-3中发现的新型硫代硫酸盐氧化途径，其为ZVS的形成提供了新的线索。使用电子显微镜，能量分散和拉曼光谱来证实瑟。 Flavus21-3有效地将硫代硫酸盐转化为ZVS。我们接下来使用组合的蛋白质组学和遗传方法来鉴定硫代硫酸盐脱氢酶（TSDA）和硫代烃（SOXB）在将硫代硫酸盐转化为ZVS中的关键作用。不同硫中间体的化学计量结果进一步阐明了TSDA在将硫代硫酸酯转化为四分离化物（-O3S-SS-SO3-）的功能，从四分制中释放硫酮中的硫酮以形成ZV和硫酸二氧化基团（SDOA / SDOB）以氧化ZVs至亚硫酸盐一些条件。值得注意的是，TSDA，SOXB和SDOO / SDOB的同源物在细菌中广泛存在，包括来自不同环境的inerythrobackspecies。这强烈表明，这种新的硫代硫酸盐氧化途径可能经常被微生物使用，在生物地良细化学硫循环中起重要作用。

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    Zhang Jing; Liu Rui; Xi Shichuan; Cai Ruining; Zhang Xin; Sun Chaomin;

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    Chinese Acad Sci CAS Key Lab Expt Marine Biol Inst Oceanol Qingdao Peoples R China;

    Chinese Acad Sci CAS Key Lab Expt Marine Biol Inst Oceanol Qingdao Peoples R China;

    Chinese Acad Sci Ctr Deep Sea Res Inst Oceanol Qingdao Peoples R China;

    Chinese Acad Sci CAS Key Lab Expt Marine Biol Inst Oceanol Qingdao Peoples R China;

    Chinese Acad Sci Ctr Deep Sea Res Inst Oceanol Qingdao Peoples R China;

    Chinese Acad Sci CAS Key Lab Expt Marine Biol Inst Oceanol Qingdao Peoples R China;

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  • 正文语种 eng
  • 中图分类 微生物学;
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