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首页> 外文期刊>The ISME journal emultidisciplinary journal of microbial ecology >It does not always take two to tango: 'Syntrophy' via hydrogen cycling in one bacterial cell
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It does not always take two to tango: 'Syntrophy' via hydrogen cycling in one bacterial cell

机译：通过在一个细菌细胞中通过氢气循环并不总是两次探戈：“Syntrophy”

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摘要

Interspecies hydrogen transfer in anoxic ecosystems is essential for the complete microbial breakdown of organic matter to methane. Acetogenic bacteria are key players in anaerobic food webs and have been considered as prime candidates for hydrogen cycling. We have tested this hypothesis by mutational analysis of the hydrogenase in the model acetogen Acetobacterium woodii. Hydrogenase-deletion mutants no longer grew on H-2 + CO2 or organic substrates such as fructose, lactate, or ethanol. Heterotrophic growth could be restored by addition of molecular hydrogen to the culture, indicating that hydrogen is an intermediate in heterotrophic growth. Indeed, hydrogen production from fructose was detected in a stirred-tank reactor. The mutant grew well on organic substrates plus caffeate, an alternative electron acceptor that does not require molecular hydrogen but NADH as reductant. These data are consistent with the notion that molecular hydrogen is produced from organic substrates and then used as reductant for CO2 reduction. Surprisingly, hydrogen cycling in A. woodii is different from the known modes of interspecies or intraspecies hydrogen cycling. Our data are consistent with a novel type of hydrogen cycling that connects an oxidative and reductive metabolic module in one bacterial cell, "intracellular syntrophy."
机译：缺氧生态系统中的氢转移是对甲烷的完全微生物分解的完全微生物分解至关重要。醋酸细菌是厌氧食物网中的关键球员，被认为是氢循环的主要候选者。通过模型乙酰乙酰橡皮素中的氢酶的突变分析，我们测试了这一假设。氢酶缺失突变体不再达到H-2 + CO 2或有机基材，例如果糖，乳酸或乙醇。通过向培养物加入分子氢来恢复异养生长，表明氢是异养生长的中间体。实际上，在搅拌罐反应器中检测来自果糖的氢产生。突变体在有机底物加上咖啡因种植，一种替代的电子受体，其不需要分子氢但是纳米作为还原剂。这些数据与分子氢由有机基材制备的概念一致，然后用作CO 2还原的还原剂。令人惊讶的是，A. Woodii在A的氢循环与种子或内部氢循环的已知模式不同。我们的数据与一种新型类型的氢循环一致，可在一个细菌细胞中连接氧化和还原代谢模块，“细胞内的同步”。

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