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首页> 外文期刊>The Journal of Nutritional Biochemistry >Higher insulin sensitivity in EDL muscle of rats fed a low-protein, high-carbohydrate diet inhibits the caspase-3 and ubiquitin-proteasome proteolytic systems but does not increase protein synthesis
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Higher insulin sensitivity in EDL muscle of rats fed a low-protein, high-carbohydrate diet inhibits the caspase-3 and ubiquitin-proteasome proteolytic systems but does not increase protein synthesis

机译：喂养低蛋白质的大鼠EDL肌肉中胰岛素敏感性较高，高碳水化合物饮食抑制了Caspase-3和泛素 - 蛋白酶体蛋白水解系统，但不会增加蛋白质合成

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Compared with the extensor digitorum longus (EDL) muscle of control rats (C), the EDL muscle of rats fed a low-protein, high-carbohydrate diet (LPHC) showed a 36% reduction in mass. Muscle mass is determined by the balance between protein synthesis and proteolysis; thus, the aim of this work was to evaluate the components involved in these processes. Compared with the muscle from C rats, the EDL muscle from LPHC diet-fed rats showed a reduction (34%) in the in vitro basal protein synthesis and a 22% reduction in the in vitro basal proteolysis suggesting that the reduction in the mass can be associated with a change in the rate of the two processes. Soon after euthanasia, in the EDL muscles of the rats fed the LPHC diet for 15 days, the activity of caspase-3 and that of components of the ubiquitin-proteasome system (atrogin-1 content and chymotrypsin-like activity) were decreased. The phosphorylation of p70(S6K) and 4E-BP1, proteins involved in protein synthesis, was also decreased. We observed an increase in the insulin-stimulated protein content of p-Akt. Thus, the higher insulin sensitivity in the EDL muscle of LPHC rats seemed to contribute to the lower proteolysis in LPHC rats. However, even with the higher insulin sensitivity, the reduction in p-E4-BP1 and p70(S6K) indicates a reduction in protein synthesis, showing that factors other than insulin can have a greater effect on the control of protein synthesis. (C) 2016 Elsevier Inc. All rights reserved.
机译：与对照大鼠（C）的伸肌位数（EDL）肌肉相比，喂养低蛋白质高碳水化合物饮食（LPHC）的大鼠EDL肌肉显示出质量降低36％。肌肉质量由蛋白质合成和蛋白水解之间的平衡决定;因此，这项工作的目的是评估这些过程中涉及的组件。与C大鼠的肌肉相比，来自LPHC饮食喂养大鼠的EDL肌肉在体外基础蛋白质合成中表现出还原（34％），体外基础蛋白质的减少22％，表明质量减少与两个过程的速率的变化相关联。 Euthanasia不久，在喂养LPHC饮食的大鼠的EDL肌肉中15天，Caspase-3的活性降低了泛素 - 蛋白酶体系（奥特蛋白-1含量和胰凝乳蛋白样活性）的活性。 P70（S6K）和4E-BP1，参与蛋白质合成的蛋白质的磷酸化也降低。我们观察到胰岛素刺激的p-akt蛋白质含量的增加。因此，LPHC大鼠EDL肌肉中较高的胰岛素敏感性似乎有助于LPHC大鼠的较低蛋白水解。然而，即使胰岛素敏感性较高，P-E4-BP1和P70（S6K）的还原也表明蛋白质合成的降低，表明除胰岛素以外的因素可以对蛋白质合成的控制具有更大的影响。 （c）2016年Elsevier Inc.保留所有权利。

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