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首页> 外文期刊>The Journal of Prosthetic Dentistry >Influence of recasting different types of dental alloys on gingival fibroblast cytotoxicity
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Influence of recasting different types of dental alloys on gingival fibroblast cytotoxicity

机译：重铸不同类型牙科合金对牙龈成纤维细胞毒性的影响

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Surplus alloy from the initial casting is commonly reused with the addition of new alloy. This recasting procedure could affect the cytotoxicity of dental alloys. The purpose of this in vitro study was to evaluate the effect of repeated casting of high-noble and base metal alloys on gingival fibroblast cytotoxicity. Disk-shaped specimens (5 × 2 mm, n=60) of a high-noble (Au-Pt) and 2 base metal (Ni-Cr and Cr-Co, n=20) alloys were prepared with 100 new alloy and 50, 65, and 100 once recast alloy. The elemental composition of specimens was analyzed with X-ray energy-dispersive spectroscopy. Five specimens from each group were conditioned in saline with 3 fetal bovine serum albumin. The conditioning media were analyzed for elemental release with atomic absorption spectroscopy. Cytotoxic effects were assessed on human gingival fibroblast with a 3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyl tetrazolium bromide (MTT) colorimetric assay. The data were analyzed with 1-way and 2-way ANOVA and Tukey's HSD multiple comparison test (α-=.05). Elemental compositions of Co-Cr and Au-Pt alloys were significantly different among casting protocols. Elemental release of Co-Cr and Ni-Cr alloys was significantly different between new and recast specimens (P<.001). Nickel release increased with recast alloy addition. The 2-way ANOVA showed a significant effect of the casting procedure (P<.001) alloy group (P<.001) and their interaction for cytotoxicity (P<.001). The Ni-Cr alloy groups with 65 and 100 recast alloy had lower cellular activity than all other specimens (P<.001). The results of this study indicated that alloys containing nickel have increased cytotoxic effects and that composition of the alloys affected the cytotoxicity. Furthermore, recasting nickel-containing alloys with 65 surplus metal addition significantly increased the cytotoxic activity.
机译：来自初始铸件的剩余合金通常随着新合金的添加而重复使用。这种重铸过程可能影响牙科合金的细胞毒性。这种体外研究的目的是评估重复浇注高贵金属和贱金属合金对牙龈成纤维细胞细胞毒性的影响。用100个新合金和50个，制备高贵巴（AU-PT）和2个贱金属（Ni-Cr和Cr-Co，N = 20）合金的磁盘状样品（5×2mm，n = 60）合金。 ，65和100一旦重铸合金。用X射线能量分散光谱分析标本的元素组成。来自每组的五个标本用3个胎儿牛血清白蛋白在盐水中调节。分析调节培养基以用原子吸收光谱分子释放进行元素释放。用3-（4.5-二甲基噻唑-2-基）-2.5-二苯基四唑溴（MTT）比色测定对人牙龈成纤维细胞评估细胞毒性作用。通过单向和双向ANOVA和Tukey的HSD多比较测试（α - = 05）分析数据。 CO-Cr和Au-Pt合金的元素组合物在铸造方案中具有显着差异。 CO-Cr和Ni-Cr合金的元素释放在新的和重量标本之间具有显着差异（P <.001）。镍释放用重量合金添加增加。双向ANOVA显示出铸造程序（P <.001）合金组（P <.001）的显着效果及其对细胞毒性的相互作用（P <.001）。 Ni-Cr合金基团具有65和100重量合金的细胞活性比所有其他样品更低（P <.001）。该研究的结果表明，含镍的合金具有增加的细胞毒性作用，并且该合金的组成影响了细胞毒性。此外，用65个剩余金属添加具有65个剩余金属的含镍的合金显着增加了细胞毒性活性。

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    ImirzaliogluP.; AlaaddinogluE.; YilmazZ.; OduncuogluB.; YilmazB.; RosenstielS.;

  • 作者单位

    Department of Prosthodontics Ba?kent University School of Dentistry Ankara Turkey;

    Department of Periodontology Ba?kent University School of Dentistry 1. Cadde 11. Sokak No:26;

    Department of Genetics Ba?kent University School of Medicine Ankara Turkey;

    Department of Periodontology Ba?kent University School of Dentistry Ankara Turkey;

    Division of Restorative and Prosthetic Dentistry Ohio State University College of Dentistry;

    Division of Restorative and Prosthetic Dentistry Ohio State University College of Dentistry;

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  • 正文语种 eng
  • 中图分类 口腔科学;
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