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首页> 外文期刊>The American journal of Chinese medicine >Effects of Celastrus orbiculatus on Epithelial Mesenchymal Transition in Gastric Mucosal Epithelial Cells by Inhibiting Lgr5 Expression from Rats with Gastric Precancerous Lesions
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Effects of Celastrus orbiculatus on Epithelial Mesenchymal Transition in Gastric Mucosal Epithelial Cells by Inhibiting Lgr5 Expression from Rats with Gastric Precancerous Lesions

机译：CelaStrus Orbiculatus对胃粘膜上皮细胞上皮间充质转换的影响，抑制胃癌癌前病变的LGR5表达

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摘要

The extract of Celastrus orbiculatus (COE) has been shown to possess anti-Helicobacter pylori (H. pylori) activity and anticancer effects in vitro and in vivo. However, the molecular mechanism by which COE on precancerous lesions of gastric cancer (PLGC) has not been fully elucidated so far. The purpose of this study is to evaluate the effect and mechanism of COE in the rat model of PLGC, after the rat model of PLGC was successfully constructed. The effects of COE in gastric mucosa of rats with PLGC were tested using routine pathology and a transmission electron microscope (TEM) analysis. The protein and mRNA expression levels of epithelial mesenchymal transition (EMT) markers (E-cadherin, N-cadherin and Vimentin) and leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5) were detected adopting techniques of immunohistochemistry (IHC), realtime PCR (RT-PCR) and western blot assays. The body weight of PLGC rats was significantly higher in the COE group than that in the untreated group. The process of PLGC was significantly reversed after COE treatment, shown by observing the changes of histopathological morphology and ultrastructure. Gastric mucosal epithelial cells in COE high dose (COE-H) group showed significantly higher expression levels of E-cadherin, and lower expression levels of N-cadherin, Vimentin and Lgr5 than those of the untreated group. COE could suppress the spatial distribution of Lgr5+ cell changes in PLGC rats. These findings suggested that the therapeutic mechanisms of COE in treating PLGC might be related with its effects on reversing the EMT process and inhibiting Lgr5 expression.
机译：Celastrus Orbiculatus（COE）的提取物已被证明在体外和体内具有抗幽门螺杆菌（H.Pylori）活性和抗癌作用。然而，到目前为止，COE对胃癌（PLGC）的癌前病变的分子机制尚未完全阐明。本研究的目的是评估COE在PLGC大鼠成功构建之后PLGC大鼠模型的效果和机制。使用常规病理学和透射电子显微镜（TEM）分析测试用PLGC大鼠大鼠胃粘膜胃粘膜的影响。上皮间充质转变（EMT）标记（E-Cadherin，N-Cadherin和Vimentin）和富含富含富含含有富含含量的含有含少的重复的G-蛋白偶联受体5（LGR5）的蛋白质和mRNA表达水平被免疫组织化学技术（IHC ），实时PCR（RT-PCR）和Western印迹测定。 COE组的PLGC大鼠的体重显着高于未处理基团的大鼠。 COE处理后PLGC的方法显着逆转，通过观察组织病理学形态和超微结构的变化而显示。 COE高剂量（COE-H）组中的胃粘膜上皮细胞显示出高于E-Cadherin，N-Cadherin，Vimentin和LGR5的表达水平明显高于未处理基团的表达水平。 COE可以抑制PLGC大鼠LGR5 +细胞变化的空间分布。这些发现表明，COE在治疗PLGC中的治疗机制可能与其对逆转EMT过程和抑制LGR5表达的影响有关。

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