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首页> 外文期刊>The Pediatric infectious disease journal >Microbiota of Children With Complex Appendicitis Different Composition and Diversity of The Microbiota in Children With Complex Compared With Simple Appendicitis
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Microbiota of Children With Complex Appendicitis Different Composition and Diversity of The Microbiota in Children With Complex Compared With Simple Appendicitis

机译：具有复杂的阑尾炎的儿童微生物群不同的组成和多元化的微生物的多样性，与简单的阑尾炎相比

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Background: Two types of appendicitis are hypothesized, simple and complex, with potential different treatment strategies. To improve differentiation, underlying pathogeneses need to be further unraveled. Aim: To determine if the microbial composition in the appendix differs between children with simple and complex appendicitis. Methods: Two-center, prospective cohort study including 40 children (0-17 years old) undergoing appendectomy for suspected appendicitis. Appendix tissue was used for IS-pro analysis to identify bacterial species by their length of 16S-23S rDNA interspacer (IS) region. Cluster analysis, based on IS-profiles, and correspondence with type of appendicitis, using Fisher exact test, was performed. Simple and complex appendicitis were compared regarding bacterial presence, intensity and diversity, using Fisher exact test and Mann-Whitney U test, respectively. Results: Appendicitis was confirmed in 36 of 40 patients (16 simple, 20 complex). Cluster analysis identified 2 clusters, encompassing 34 patients. Distribution of simple and complex appendicitis was 12 (80%) and 3 (20%) versus 3 (16%) and 16 (84%) patients for clusters 1 and 2, respectively (P < 0.001). Complex appendicitis was on phylum level characterized by an increased intensity (Bacteroidetes P = 0.001, Firmicutes, Actinobacteria, Fusobacteria and Verrucomicrobia (FAFV) P = 0.005 and Proteobacteria P < 0.001) and diversity (Bacteroidetes P = 0.001 and Proteobacteria P = 0.016) and an increased abundance of 5 species (Alistipes finegoldii P = 0.009, Bacteroides fragilis P = 0.002, Escherichia coli P = 0.014, Parvimonas micra P = 0.022 and Sutterella spp P = 0.026). Conclusions: The microbial composition of the appendix differs between children with simple and complex appendicitis, regarding both composition and diversity. Future research should focus on the role of these bacteria in the pathogenesis of both types and its implications for preoperative diagnostics.
机译：背景：两种类型的阑尾炎是假设的，简单和复杂的，具有潜在的不同治疗策略。为了改善分化，需要进一步解开潜在的病原因。目的：确定附录中的微生物组合物是否与具有简单和复杂的阑尾炎的儿童之间的不同。方法：双中心，前瞻性队列研究，包括40名儿童（0-17岁）接受疑似阑尾炎的阑尾切除术。附录组织用于IS-Pro分析，以鉴定细菌种类的长度为16s-23s rdna Interspacer（IS）区域。基于IS-型谱的聚类分析和使用FISHER精确测试的与阑尾炎类型的对应性进行进行。比较简单和复杂的阑尾炎，与细菌存在，强度和多样性分别使用Fisher精确测试和Mann-Whitney U测试。结果：40例患者中36例（16例，20个复杂）确认了阑尾炎。集群分析确定了2个簇，包括34名患者。简单且复杂的阑尾炎的分布分别为12（80％）和3（20％），分别为3（16％）和16例（16％）和16名（84％）患者（P <0.001）。复杂的阑尾炎是在体内水平上表征的，其强度增加（菌体P = 0.001，Fassicutes，Actinobacteria，Fusobacteria（FAFV）P = 0.005和Proteobacteria P <0.001）和多样性（Bacteropetes P = 0.001和Proteobacteria P = 0.016）和5种的丰度增加（alistipes finegoldii p = 0.009，菌叶片p = 0.002，大肠杆菌p = 0.014，parvimonas micra p = 0.022和stutterla spp p = 0.026）。结论：关于组成和多样性，具有简单和复杂的阑尾炎的儿童之间的微生物组成。未来的研究应专注于这些细菌在两种类型发病机制中的作用及其对术前诊断的影响。

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