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Fiber-optic enzyme biosensor for direct determination of organophosphatenerve agents

机译：光纤酶生物传感器直接测定有机磷酸神经药

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摘要

A fiber-optic enzyme biosensor for the direct measurement of organophosphate nerve agents was developed. The basic element of this biosensor is organophosphorus hydrolase immobilized on a nylon membrane and attached to the common end of a bifurcated optical fiber bundle. The enzyme catalyzes the hydrolysis of organophosphate compounds to form stoichiometric amounts of chromophoric products that absorb light at specific wavelengths. The back-scattered radiation of the specific incident radiation was measured using a photomultiplier detector and correlated to the organophosphate concentration. The effects of buffer pH, temperature, and the units of enzyme immobilized on the steady-state and kinetic response of the biosensor were investigated to optimize the operating conditions for the fiber-optic enzyme biosensor. These conditions were then used to measure parathion, paraoxon, and coumaphos selectively without interference from carbamates and triazines. Concentrations as low as 2 mu M can be measured in less than 2 min using the kinetic response. When stored in buffer at 4 degrees C the biosensor shows long-term stability.
机译：开发了一种用于直接测量有机磷酸神经药的光纤酶生物传感器。这种生物传感器的基本元素是有机磷水解酶，固定在尼龙膜上并附着在分叉光纤束的公共端。该酶催化有机磷酸酯化合物的水解，形成化学计量的发色产物，可吸收特定波长的光。使用光电倍增管检测器测量特定入射辐射的反向散射辐射，并将其与有机磷酸盐浓度相关。研究了缓冲液pH，温度和固定化酶的单位对生物传感器的稳态和动力学响应的影响，以优化光纤酶生物传感器的操作条件。然后将这些条件用于选择性地检测对硫磷，对氧磷和苯丙胺，而不受氨基甲酸酯和三嗪的干扰。使用动力学响应，可以在不到2分钟的时间内测量出低至2μM的浓度。当将生物传感器存储在4摄氏度的缓冲液中时，它显示出长期稳定性。

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