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On-line fluorescence profile of aerobic sludge digestion

机译：好氧污泥消化的在线荧光图

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An online fluorometer designed for following intracellular NAD(P)H was used to monitor aerobic sludge digestion experiments. The fluorescence showed an initial rise to a high plateau, a sharp decline after staying at the plateau for 20-60 h, and a trailing very slow decrease. The characteristic fluorescence profile was shown to result mainly from the solids-associated fluorescence, after ruling out other factors such as pH, temperature, and supernatant fluorescence. The fluorescence profile was, however, not a mere result of the decreasing solids concentration. The varying sludge viability and population composition (e.g., the decay of heterotrophs and the increasing fraction of nitrifiers) played important roles. The fluorescence profile correlated well with the profile of the viable heterotrophic cell number concentration evaluated with TSB-agar plates. The initial increase of the number concentration was attributed to the growth of multiple small bacteria from the lysate of each large microorganism, which was demonstrated in the experiments with baker's yeast as the starting culture for digestion. The fluorescence profiles observed in the yeast experiments were similar to those in the sludge experiments. Responding to glucose additions and the switch from aerobic to anaerobic conditions, the yeast systems showed typical step increases of fluorescence as expected from the change of NAD(PH level associated with heterotrophic metabolism. However, no such fluorescence responses were detectable in the sludge digestion systems. NAD(P)H were thus uncertain to be responsible for the online fluorescence observed. Nonetheless, the initial fluorescence plateau corresponded to the period of rapid digestion and, for the plant studied, the EPA regulation criteria of VSS reduction >38% and/or SOUR <1.5 mg of O-2 (g of TS)(-1) h(-1) were satisfied at the end of the plateau. The online fluorescence provides an effective means of monitoring the aerobic sludge digestion process.
机译：设计用于跟踪细胞内NAD（P）H的在线荧光计用于监测好氧污泥消化实验。荧光显示出最初的上升到高平台，在平台上停留20-60小时后急剧下降，并且下降非常缓慢。在排除其他因素（例如pH，温度和上清液荧光）后，显示出特征性荧光曲线主要由与固体相关的荧光产生。然而，荧光分布图不仅仅是固体浓度降低的结果。污泥的生存能力和种群组成的变化（例如，异养菌的衰变和硝化剂分数的增加）起着重要作用。荧光图与用TSB-琼脂平板评估的存活异养细胞数浓度的图很好地相关。数量浓度的最初增加归因于每种大微生物裂解物中多种小细菌的生长，这在以面包酵母作为消化起始培养物的实验中得到了证明。在酵母实验中观察到的荧光图与污泥实验中的相似。响应葡萄糖添加和从有氧到厌氧条件的转变，酵母系统显示出典型的逐步荧光增加，这是由于NAD（PH水平与异养代谢相关）所预期的，但是在污泥消化系统中未检测到此类荧光反应。因此，不确定NAD（P）H是否会引起在线荧光现象，尽管如此，初始荧光平稳期对应于快速消化的时期，并且对于所研究的植物，EPA调节标准中VSS的减少> 38％和/或在高原期末满足SOUR <1.5 mg O-2（g TS）（-1）h（-1）的要求，在线荧光提供了一种监测好氧污泥消化过程的有效手段。

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