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首页> 外文期刊>Biotechnology Progress >pH-sensitive hydrogels as gastrointestinal tract absorption enhancers:transport mechanisms of salmoncal citonic and other model molecules using the caco-2 cell model
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pH-sensitive hydrogels as gastrointestinal tract absorption enhancers:transport mechanisms of salmoncal citonic and other model molecules using the caco-2 cell model

机译：pH敏感水凝胶作为胃肠道吸收促进剂：使用caco-2细胞模型的鲑鱼子柠檬酸和其他模型分子的转运机制

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The maininterest of this work was the investigation of the transport mechanisms of salmoncalciton in through te epithelial cell monolyae rin the presence and absence of pH-sensititive hydrogel nanospheres composed of poly(methacrylic acid-grafted-poly(ethylene glycol)(PMAA-g-EG).For this purpsoe,a gastrointestinal cell culture model,the Caco-2 cell line,was employed.The transport of other macromolecules such as fluorescein sodium,fluoresce in isothiocyanate dextran,and ~(14)C-mannitol were also investigated and compared.Transpor texperiments were conducted in the apical-to-basolateral direction at 37 and 5degC and from the basolateral-to-apical direction at 37degC.Results revealed that the presence of P(MAA-g-EG)nanospheres increased the transport of paracellularly transported molecules such as ~(14)C-mannitol and fluorescein isothiocyanate dextran when compared to controls.Fluoresce in sodium salt solutions were investigated as an actively transported moelcule.The transport of fluorescein was affected by the concentration of PEG chains in the structure.Salmon calciton in transport was enhanced in the presence of the nanospheres.Thecomparison of the transport behavior of dextran and calciton inrevealed that the maintransport mechanism for salmoncalciton in through epithelial cell monolayers is predominantly paracellular.
机译：这项工作的主要目的是研究鲑鱼降钙素在上皮细胞单层单胞菌中的转运机制，研究是否存在由甲基丙烯酸接枝的聚乙二醇（PMAA-g-）组成的pH敏感水凝胶纳米球。 EG）。为此，我们使用了胃肠道细胞培养模型Caco-2细胞系。还研究了其他大分子（例如荧光素钠，异硫氰酸右旋糖酐中的荧光素和〜（14）C-甘露醇）的转运。在37°C和5°C时从顶部到基底外侧方向以及在37°C时从基底外侧到顶部方向进行穿膜实验。结果表明，P（MAA-g-EG）纳米球的存在增加了细胞旁细胞的转运。与对照相比，可以转运〜（14）C-甘露糖醇和异硫氰酸荧光素右旋糖苷等分子。研究了钠盐溶液中的氟作为一种主动转运的分子。 cein受到结构中PEG链浓度的影响。存在纳米球时，鲑鱼降钙素的运输增强。右旋糖酐和降钙素的运输行为的比较表明，鲑鱼钙化素通过上皮细胞单层的主要运输机制主要是旁细胞。 。

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