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Optimization of high molecular weight pullulan production by aureobasiduium pullulans in batch fermentations

机译:金黄色葡萄球菌分批发酵生产高分子量支链淀粉的优化

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摘要

Of five strains of Aureobasidium pullulans studied,NRRLY-2311 -1 yielded the highest titer (26.2 g/L) of pullulan and formed the lsoest amount of melanin-like pigment.Sucrose was superior to glucose as the carbon and energy source on the basis of yield and titer of pullulan produced.Pullulantiter was higher (26.2 vs 5.1 g/L),biomass concentration was lower (6.9 vs 12.7 g/L),and DO was lower (0 vs 60% of saturation) when the fermenter was agitated by a marine propeller compared to Rushton impellers.Pullulan produced by strain NRRL Y-2311-1 ranged in weight-average molar mass (M_w) from 486 KDa and number-average molar mass (M_n) from 220 Da on day 1 of growth to 390 KDa and 690Da on day 6;M_w declined by about 35% from day 1 today 3,the day of maximum pullalan titer.For theother strains,the ranges of molar mass on the day of maximum pullulantiter were 338-614 KDa,(M_w) and 100-6820 Da (M_n).
机译:在研究的5株金黄色葡萄球菌中,NRRLY-2311 -1的滴度最高(26.2 g / L),并形成了数量最少的黑色素样色素。蔗糖优于葡萄糖作为碳和能源的基础发酵罐搅拌时,Pululantiter较高(26.2 vs 5.1 g / L),生物量浓度较低(6.9 vs 12.7 g / L),DO较低(0 vs 60%饱和度)。 NRRL Y-2311-1菌株产生的Pullulan在生长的第1天至第1天的重均摩尔质量(M_w)从486 KDa不等,数均摩尔质量(M_n)从220 Da不等。第6天为390 KDa和690Da;从今天的第1天第3天即最大普鲁兰滴度开始,M_w下降了约35%。对于其他菌株,最大普鲁兰特滴度当日的摩尔质量范围为338-614 KDa,(M_w )和100-6820 Da(M_n)。

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