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首页> 外文期刊>Biotechnology Progress >Influence of fungal elicitors onproductionof ajmalicine by cell cultures of catharanthus roseus
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Influence of fungal elicitors onproductionof ajmalicine by cell cultures of catharanthus roseus

机译：长春花的细胞培养对真菌诱导剂的影响

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Suspension cultures of Catharanthus roseus (C.roseus) were elicited with fungal cell wall fragments of Aspergillus niger (A.niger),Fusarium moniliforme (F.moniliforme), and Trichoderma viride (T.viride).The effects of elicitor dosage, exposures time, and age of subculture on ajmalicine accumulation were studied. A higher concentration of elicitor extract responded positively to C.roseus suspension cultures. Ajmalicine accumulation increased by about 3-fold when cells were treated with A.niger, F.moniliforme, and T.viride. The maximum ajmalicine production (75 mug g~(-1) dry weight (DW) was observed in cells treated with T.viride. Cell cultures were elicited with 5% preparation of A.niger, F.moniliforme, and T.viride and exposed for 24, 48, 72, and 96 h for elicitation. Suspension cultures elicited with T.viride for 48 h showed a 3-fold increase (87 mug g~(-1) DW) in ajmalicine contents, whereas A.niger and F.moniliforme synthesized a 2-fold increase in alkaloid and yielded 52 and 56 mug g~(-1) DW ajmalicine, respectively.C.roseus cells of different age (5, 10, 15, 20 and 25 days old) were treated with a 5% elicitor of A.niger, F.moniliforme, and T.viride and investigated elicitors activity at different age of cell cultures. Maximum yield 166 mug g~(-1) DW of ajmalicine was synthesized in 20 day old suspension cultures treated with T.viride. A longer period of incubation of cell cultures with elicitors adversely affected the ajmalicine synthesis.
机译：用黑曲霉（A.niger），mon镰孢（F.moniliforme）和木霉（Trichoderma viride）（T.viride）的真菌细胞壁碎片诱导长春花（C.roseus）的悬浮培养。诱导剂剂量，暴露量的影响研究了次培养的时间和年龄对阿马利菊酯积累的影响。较高浓度的激发子提取物对玫瑰色念珠菌悬浮培养物呈阳性反应。当用黑曲霉，念珠菌和T.viride处理细胞时，阿马利碱的积累增加了约3倍。在用T.viride处理的细胞中观察到最大的苯丙胺生产量（75马克杯（-1）干重（DW）。用5％的A.niger，F.moniliforme和T.viride制备制剂诱导细胞培养。暴露24、48、72和96 h进行诱导;用T.viride诱导的悬浮培养物48 h的苦参碱含量增加了3倍（87杯g〜（-1）DW），而A.niger和F.moniliforme合成的生物碱增加了2倍，分别产生了52和56马克杯g〜（-1）DW阿马利菊碱。处理了不同年龄（5、10、15、20和25天龄）的玫瑰色C.roseus细胞以5％的A.niger，F.moniliforme和T.viride激发子为研究对象，并研究了在不同细胞培养年龄下激发子的活性，在20天的悬浮培养物中合成了最大产量166杯g〜（-1）DJ的阿马利菊酯用T.viride处理过的细胞与激发子一起培养的时间较长，会影响阿马利辛的合成。

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