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Stationary and microcarrier cell culture processes for propagating Japanese encephalitis virus

机译：传播日本脑炎病毒的固定和微载体细胞培养方法

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摘要

Inactivated mouse-brain-derived vaccines for Japanese encephalitis virus (JEV) have been used for many years. Recently, attempts have been made to employ cultured Vero cells to replace mouse brain tissues for developing cell-culture-derived vaccines that will be more suitable for worldwide usage. In this study, JEV replication processes in Vero and BHK cells and between stationary and microcarrier culture systems were investigated. Our results demonstrated that a stationary Vero cell culture system produced higher viral titers of JEV, including the Beijin-1 vaccine strain and the attenuated strain CH2195LA, than microcarrier culture did. BHK cells showed less significant differences in their replication kinetics between stationary and microcarrier cultures. Reducing serum concentration during infection led to an overall decrease of JEV production in Vero cells but an increase in BHK cells. By establishing a complete serum-free Vero cell culture, the microcarrier system resulted in a more than 4-log lowered yield compared to that of the stationary culture for JEV production. Thus, the stationary culture is the most efficient system for JEV production from cultured Vero cells.
机译：日本脑炎病毒（JEV）的灭活小鼠脑源疫苗已经使用了很多年。近来，已经尝试使用培养的Vero细胞代替小鼠脑组织以开发源自细胞培养的疫苗，该疫苗将更适合全世界使用。在这项研究中，研究了在Vero和BHK细胞以及固定和微载体培养系统之间JEV复制过程。我们的结果表明，与微载体培养相比，固定的Vero细胞培养系统产生的JEV病毒滴度更高，包括Beijin-1疫苗株和减毒株CH2195LA。 BHK细胞在固定和微载体培养之间的复制动力学方面显示出较小的显着差异。在感染过程中降低血清浓度可导致Vero细胞中JEV的产生总体减少，但BHK细胞则增加。通过建立完整的无血清Vero细胞培养物，与用于JEV生产的固定培养物相比，微载体系统的产量降低了4对数以上。因此，固定培养是从培养的Vero细胞生产JEV的最有效系统。

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