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首页> 外文期刊>Thorax: The Journal of the British Thoracic Society >Expression of growth factors and remodelling of the airway wall in bronchial asthma.
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Expression of growth factors and remodelling of the airway wall in bronchial asthma.

机译：支气管哮喘气道壁的生长因子和重塑的表达。

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BACKGROUND: Bronchial asthma is characterised by airway structural changes, including mucosal inflammatory infiltration and subepithelial collagen deposition, that may represent the morphological basis for the chronicity of the disease. The relationship between airway wall thickness and growth factors in asthma has not been elucidated. METHODS: Bronchial biopsy specimens were obtained from 21 asthmatic patients and eight healthy subjects and the basement membrane thickness was measured by light microscopy and electron microscopy. At the same time the numbers of eosinophils and fibroblasts were assessed and the expression of transforming growth factor beta 1 (TGF-beta 1), platelet derived growth factor (PDGF), and insulin like growth factor (IGF) I in the bronchial mucosa was examined by immunostaining. The relationship between the degree of thickening of the subepithelial layer and both the clinical data and pulmonary function were also investigated. RESULTS: The basement membrane of the asthmatic patients was thicker than that of the healthy controls (median 8.09 versus 4.02 microns). Electron microscopic examination of the basement membrane revealed thickening of the subepithelial lamina reticularis; this thickening significantly correlated with the number of fibroblasts in the submucosa in the asthmatic subjects (rs = 0.88) but not in the controls (rs = 0.70). There was a significantly higher number of eosinophils in the airways of the asthmatic subjects than in the healthy subjects (EG1 + cells: 52.0 versus 2.0/mm2, EG2 + cells: 56.0 versus 1.5/mm2). The expression of each growth factor in the bronchial mucosa was similar in asthmatic and healthy subjects (TGF-beta 1: 18.0% versus 16.0%, PDGF: 37.0% versus 32.5%, IGF-I: 15.0% versus 8.0%). A weak but statistically significant correlation was found between the number of fibroblasts and the expression of TGF-beta 1 in asthmatic subjects (rs = 0.50). There was a significant correlation between the thickness of the subepithelial layer in asthmatic subjects and the attack score (rs = 0.58) and a significant inverse correlation between the subepithelial collagen thickness in asthmatic subjects and airway hypersensitivity (rs = -0.65). CONCLUSIONS: These findings indicate that the thickening of the subepithelial layer in bronchial asthma is due to an increase in fibroblasts, and that the thickness of the subepithelial collagen appears to be linked to an increase in bronchial responsiveness and exacerbation of clinical manifestations.
机译：背景：支气管哮喘的特征在于气道结构变化，包括粘膜炎症浸润和龈下性胶原蛋白沉积，这可能代表疾病的慢性慢性的形态学依据。哮喘气道壁厚与生长因子之间的关系尚未阐明。方法：通过光学显微镜和电子显微镜测量来自21例哮喘患者和八个健康受试者的支气管活检标本，并通过光学显微镜和电子显微镜测量基底膜厚度。同时评估嗜酸性粒细胞和成纤维细胞的数量，并表达转化生长因子β1（TGF-β1），血小板衍生的生长因子（PDGF）和支气管粘膜中的生长因子（IGF）I等胰岛素是通过免疫染色检查。还研究了耻骨上层的增厚程度与临床数据和肺功能之间的关系。结果：哮喘患者的基底膜比健康对照较厚（中位数8.09对4.02微米）。基础膜的电子显微镜检查显示耻骨上薄层旋转膜的增厚;这种增厚与哮喘受试者中粘膜下的成纤维细胞数显着相关（Rs = 0.88），但不在对照中（Rs = 0.70）。在哮喘受试者的气道中存在比在健康受试者（EG1 +细胞：52.0对2.0 / mm 2，EG2 +细胞与1.5 / mm2）中的哮喘受试者的嗜酸虫细胞中有明显较高的嗜酸性粒细胞。支气管粘膜中每种生长因子的表达在哮喘和健康受试者中相似（TGF-β1：18.0％对16.0％，PDGF：37.0％与32.5％，IGF-I：15.0％对8.0％）。在哮喘受试者中成纤维细胞的数量和TGF-β1的表达之间发现了弱但统计学上的相关性（Rs = 0.50）。在哮喘受试者中的耻骨层的厚度和攻击评分（Rs = 0.58）之间存在显着的相关性，并且诸如哮喘受试者和气道超敏反应中的龈皮细胞胶原厚度之间的显着逆相关性（Rs = -0.65）。结论：这些发现表明，支气管哮喘中耻骨层的增厚是由于成纤维细胞的增加，并且蜂上皮胶原的厚度似乎与支气管反应性和临床表现的加剧的增加。

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