Macroaffinity Ligand-Facilitated Three-Phase Partitioning (MLFTPP) of alpha-Amylases Using a Modified Alginate

Kalyani Mondal; Aparna Sharma; Lata; Munishwar Nath Gupta

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Macroaffinity Ligand-Facilitated Three-Phase Partitioning (MLFTPP) of alpha-Amylases Using a Modified Alginate

机译：Macroaffinity配体促进的使用改性藻酸盐的α-淀粉酶的三相划分（MLFTPP）

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The cruce extracts of alpha-amylases when mixed with alginate,tert-butyl alcohol,and ammonium sulfate resulted in an interfactal precipitate contining polymer-bound amylase.The precipitate was dissolved in 1 M maltose to recover alpha-amylase activity The recovery of alpha-amylases were 74% 77% and 92% in the case of Bacillus amyloliquefaciens,wheat germ and porcine pancreas,respecitively.All purified preparations showed a single band on SDS-PAGE

机译：将α-淀粉酶的粗提取物与藻酸盐，叔丁醇和硫酸铵混合后，会形成一种分子间沉淀物，继续与聚合物结合的淀粉酶。将沉淀物溶于1 M麦芽糖中以恢复α-淀粉酶活性。淀粉芽孢杆菌，小麦胚芽和猪胰腺的淀粉酶分别为74％77％和92％。所有纯化的制剂在SDS-PAGE上均显示一条条带

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《Biotechnology Progress》 |2003年第2期|共2页
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Kalyani Mondal; Aparna Sharma; Lata; Munishwar Nath Gupta;
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正文语种 eng
中图分类生物工程学（生物技术）;分子生物学;
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1. Macroaffinity Ligand-Facilitated Three-Phase Partitioning (MLFTPP) of alpha-Amylases Using a Modified Alginate [J] . Kalyani Mondal, Aparna Sharma, Lata, Biotechnology Progress . 2003,第2期

机译：Macroaffinity配体促进的使用改性藻酸盐的α-淀粉酶的三相划分（MLFTPP）
2. Affinity Precipitation and Macroaffinity Ligand Facilitated Three-Phase Partitioning for Refolding and Simultaneous Purification of Urea-Denatured Pectinase [J] . Aparna Sharma, Ipsita Roy, Munishwar Nath Gupta Biotechnology Progress . 2004,第4期

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3. Enhancement of enzyme activity through three-phase partitioning: crystal structure of a modified serine proteinase at 1.5 A resolution. [J] . Singh RK, Gourinath S, Sharma S, Protein Engineering . 2001,第5期

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5. Controlling myoblast phenotype with RGD-modified alginate matrices. [D] . Rowley, Jon A. 2001

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Macroaffinity Ligand-Facilitated Three-Phase Partitioning (MLFTPP) of alpha-Amylases Using a Modified Alginate

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