• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Progress >Adaptation of Clostridium tyrobutyricum for Enhanced Tolerance to Butyric Acid in a Fibrous-Bed Bioreactor
【24h】

Adaptation of Clostridium tyrobutyricum for Enhanced Tolerance to Butyric Acid in a Fibrous-Bed Bioreactor

机译：酪丁酸梭菌在纤维床生物反应器中对丁酸耐受性增强的适应性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

By immobilization in a fibrous-bed bioreactor (FBB) we succeeded in adapting and selecting an acid-tolerant strain of Clostridium tyrobutyricum that can produce high concentrations of butyrate from glucose and xylose.This mutant grew well under hihg butyrate concentration(> 30 g/L) and had better fermentative ability as compared to the wild-type strain used to seed the bioreactor.Kinetic analysis of butyrate inhibition on cell groewth acid-forming enzymes,and ATPase activity showed that the adapted cells from the FBB are phydiologically different from the original wild type Compared to the wild type the adapted culature's max imum specific growth rate increased by 2.3-fold and its growth tolerance to butyrate inhibition increased by 29-fold.The dey enzymes in the butyrate-forming pathrate inhibition increased by 29-fold.The key enzymes in the butyrate-forming pathway,phosphotransbutyrylase (PTB)and butyrate kinade (BK) were also more active in the mutant with 175% higher PTB and 146% higher BK activities.Also the mutant's ATPase was less sensitive to inhibition by butyric acid as indicated by a 4-fold increase in the inhibition rate constant,and was more resistant to butyrate inhibition which also might be attributed to the higher percentage of saturated fatty acids in the membrane phospholipids (74% in the mutant vs 69% in the wild type).This study shows that cell immobilization in the FBB provides an effective means for in-process adaptation and selection of mutant with higher tolerance to inhibitory fermentation product.
机译：通过固定在纤维床生物反应器（FBB）中，我们成功地选择了耐酸的酪丁酸梭菌菌株，该菌株可从葡萄糖和木糖中产生高浓度的丁酸，该突变体在高丁酸浓度（> 30 g / L），并且与用于接种生物反应器的野生型菌株相比具有更好的发酵能力。丁酸对细胞生长酸形成酶和ATPase活性的抑制动力学分析表明，来自FBB的适应细胞在生物学上与FBB有所不同。原始野生型与野生型相比，适应的菌群的最大最大比生长速率提高了2.3倍，其对丁酸盐抑制的生长耐受性提高了29倍。形成丁酸盐的路径抑制中的dey酶提高了29倍。丁酸形成途径中的关键酶磷酸转丁酰酶（PTB）和丁酸激酶（BK）在突变体中也更具活性，PTB升高了175％，h升高了146％ BK活性较高。此外，突变体的ATPase对丁酸的抑制作用较不敏感，抑制率常数增加了4倍，表明对丁酸的抑制作用较弱，这也可能是由于饱和脂肪酸的百分比较高所致该研究表明，FBB中的细胞固定化为过程中的适应和选择对抑制性发酵产物具有更高耐受性的突变体提供了有效的手段。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号