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首页> 外文期刊>AIDS Research and Human Retroviruses >Distribution of HIV-1 infection in different T lymphocyte subsets: antiretroviral therapy-naive vs. experienced patients.
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Distribution of HIV-1 infection in different T lymphocyte subsets: antiretroviral therapy-naive vs. experienced patients.

机译：HIV-1感染在不同T淋巴细胞亚群中的分布：未接受抗逆转录病毒治疗的患者与有经验的患者。

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Memory CD4 T cells are the primary targets of HIV-1 infection, which then subsequently spreads to other T lymphocyte subsets. Antiretroviral therapy (ART) alters the pattern of HIV-1 distribution. Blood samples were collected from ART-naive or -experienced HIV-1 patients, and the memory and naive subsets of CD4(+) and CD8(+) T lymphocytes, respectively, were isolated by cell sorting. DNA was extracted and the HIV-1 env C2/V3 region PCR amplified. Amplicons were cloned and sequenced, and genetic relatedness among different HIV-1 compartments was determined by the phylogenetic analysis of clonal sequences. The viral V3 sequence of HIV-1 in each compartment was analyzed by using webPSSM to determine CCR5 or CXCR4 coreceptor binding property of the virus. The direction of viral migration among involved compartments was determined by using the MacClade program. In ART-naive patients, HIV-1 was generally confined to the memory CD4 T (mT4) cell compartment, even though in a few cases, naive CD4 T (nT4) cells were also infected. When this occurred, the HIV-1 gene migrated from nT4 to mT4. In contrast, HIV-1 was detected in nT4 and mT4 as well as in the memory CD8 T (mT8) compartments of ART-experienced patients. However, no clear pattern of directional HIV-1 gene flow among the compartments could be determined because of the small sample size. All HIV-1-infected T cell compartments housed the virus that used either CCR5 or CXCR4 as the coreceptor.
机译：记忆CD4 T细胞是HIV-1感染的主要靶标，随后会传播到其他T淋巴细胞亚群。抗逆转录病毒疗法（ART）可以改变HIV-1的分布方式。从未接受过ART或经历过HIV-1的患者中采集血样，并通过细胞分选分离出CD4（+）和CD8（+）T淋巴细胞的记忆和幼稚亚群。提取DNA并扩增HIV-1 env C2 / V3区域PCR。对扩增子进行克隆和测序，并通过克隆序列的系统进化分析确定不同HIV-1区室之间的遗传相关性。通过使用webPSSM分析每个隔室中HIV-1的病毒V3序列，以确定该病毒的CCR5或CXCR4共受体结合特性。通过使用MacClade程序确定相关区室之间的病毒迁移方向。在未接受ART治疗的患者中，HIV-1通常局限于记忆CD4 T（mT4）细胞区室，即使在少数情况下，也感染了未感染CD4 T（nT4）细胞。发生这种情况时，HIV-1基因从nT4迁移到mT4。相反，在经历过ART的患者的nT4和mT4以及记忆CD8 T（mT8）区室中检测到HIV-1。但是，由于样本量较小，无法确定各区室间定向HIV-1基因定向流动的明确模式。所有感染HIV-1的T细胞区室都装有使用CCR5或CXCR4作为共受体的病毒。

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