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首页> 外文期刊>Tissue and Cell >Osmoregulatory performance and immunolocalization of Na+/K+-ATPase in the branchiopod Artemia salina from the Sebkha of Sidi El Hani (Tunisia)
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Osmoregulatory performance and immunolocalization of Na+/K+-ATPase in the branchiopod Artemia salina from the Sebkha of Sidi El Hani (Tunisia)

机译：Sidi El Hani（突尼斯）Sebkha的Brankiopod Artemia Salina中Na + / K + -Atpase的Osmoregulatory和免疫循环化

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摘要

Artemia salina is an extremophile species that tolerates a wide range of salinity, especially hypertonic media considered lethal for the majority of other aquatic species. In this study, A. salina cysts were hatched in the laboratory and nauplii were acclimated at three different salinities (60, 139 and 212 ppt). Once in the adult phase, their hemolymph osmolality was measured. The animals were strong hypo-osmoregulators in the entire range of tested salinities, with up to 10 fold lower hemolymph osmolalities than their surrounding environment. Immunostaining of Na+/K+-ATPase was done on sections and on whole body mounts of adults in order to localize the ionocytes in different organs. An intense Na+/K+-ATPase immunostaining throughout the cells was observed in the epithelium of the ten pairs of metepipodites. A positive immunoreactivity for Na+/K+-ATPase was also detected in the maxillary glands, in the epithelium of the efferent tubule and of the excretory canal, as well as in the anterior digestive tract. This study confirms the strong hypo-osmotic capacity of this species and affords an overview of the different organs involved in osmoregulation in A. salina adults.
机译：Artemia Salina是一种极致的物种，可容忍各种盐度，特别是对于大多数其他水生物种认为致命的高渗媒体。在本研究中，A. Salina囊肿在实验室中孵化，Nauplii在三种不同的盐水（60,139和212 ppt）中适应。一旦在成人阶段，测量了它们的血淋巴渗透压。这些动物在整个测试盐度范围内是强烈的血吸虫，血淋巴渗透性高达10倍，而不是周围环境。将Na + / K + -AtPase的免疫染色在截面和成人的全身安装件上进行，以便在不同器官中定位离子细胞。在十对梅皮迪斯的上皮内观察到整个细胞中的强烈Na + / K + -ATP酶免疫染色。在颌骨腺中，在散发剂小管和排泄管的上皮中也检测到Na + / K + -AtPase的阳性免疫反应性，以及前消化道。本研究证实了该物种的强劲渗透能力，并概述了在A. Salina成年人中涉及Osmoregulation的不同器官。

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