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首页> 外文期刊>AIDS Research and Human Retroviruses >Mutational resistance pattern of HIV type 1 in CD14+ monocytes, CD4+ T cells, and plasma from treated patients.
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Mutational resistance pattern of HIV type 1 in CD14+ monocytes, CD4+ T cells, and plasma from treated patients.

机译：来自治疗患者的CD14 +单核细胞，CD4 + T细胞和血浆中HIV 1型的突变抗性模式。

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摘要

It is necessary to understand the molecular nature of the virus population that persists in cellular reservoirs. To achieve this we planned to characterize the patterns of resistance of HIV-1 in CD14(+) monocytes, CD4(+) T cells, and plasma. Blood samples were collected from 42 patients treated for HIV: 32 were in virological failure and in 10 viremia was undetectable. CD14(+) and CD4(+) T cells were isolated using magnetic beads. Genotyping of the reverse transcriptase and protease gene of HIV-1 was undertaken using the fluorescent dideoxy-terminator method. Of the 32 patients in virological failure, 24 (75%) had resistance mutations in at least one compartment. The numbers and types of mutations from monocytes were the same as those detected in both CD4(+) T cell-associated virus and plasma in only 8% whereas in 71% monocytes exhibited a different mutation pattern. In 21% of patients, the profile of drug-resistant mutations in the virus from blood monocytes was identical to that in plasma but differed from that in CD4. In the 71% of patients with virological suppression, the genotypic resistance pattern differed between monocytes and CD4(+) T cells. Circulating monocytes may harbor a viral dominant population different from those viruses circulating in blood and archived in CD4(+) T cells. Hence, monocytes and other cellular reservoirs might serve as an indirect source of a drug-resistant viral variant.
机译：有必要了解存在于细胞贮库中的病毒种群的分子性质。为实现这一目标，我们计划表征CD14（+）单核细胞，CD4（+）T细胞和血浆中HIV-1的抗性模式。从42位接受过HIV感染的患者中采集了血液样本：其中32例因病毒学衰竭而无法检测到10例病毒血症。使用磁珠分离CD14（+）和CD4（+）T细胞。 HIV-1的逆转录酶和蛋白酶基因的基因分型采用荧光双脱氧终止法进行。在病毒学衰竭的32例患者中，有24例（75％）在至少一个区室出现了耐药性突变。单核细胞的突变数量和类型与CD4（+）T细胞相关病毒和血浆中检测到的相同，仅8％，而71％的单核细胞显示出不同的突变模式。在21％的患者中，来自血液单核细胞的病毒中耐药突变的特征与血浆中相同，但不同于CD4。在71％的病毒学抑制患者中，单核细胞和CD4（+）T细胞之间的基因型耐药性模式有所不同。循环中的单核细胞可能具有病毒占优势的种群，这不同于血液中循环并存档在CD4（+）T细胞中的那些病毒。因此，单核细胞和其他细胞库可能是耐药病毒变异体的间接来源。

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