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首页> 外文期刊>AIDS Research and Human Retroviruses >HIV type 1 genetic variability in central Brazil.
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HIV type 1 genetic variability in central Brazil.

机译：巴西中部的HIV 1型遗传变异。

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This study analyzed the genes pol and env to determine the genetic variability of HIV-1 in Central Brazil. Forty-one isolates of HIV-1-infected individuals had protease, reverse transcriptase, and C2C3/ env amplified by nested PCR and sequenced. The subtype was determined by the program REGA and phylogenetic analyses. The samples identified as putative recombinant forms were analyzed by SimPlot. A high prevalence of subtype B (95.1%) was observed, followed by mosaic viruses B/F (4.9%). The amino acid sequences from 30 HIV-1 isolates were analyzed for the antigenic intrasubtype diversity. The most prevalent gp120 V3 loop motif was the GPGR (United States/Europe) (43.3%), described in B and F subtypes, followed by the GPGK tetrapeptide (10%). The Brazilian variant B" (GWGR), GFGR, and GLGR tetrapeptides were found in 6.7%. Other V3 variants were found in eight isolates (26.7%). Phylogenetic tree analysis was also performed in order to verify the relationship of the HIV-1 samples from Central Brazil with other HIV-1 sequences that circulate in Brazil. The subtype B sequences from Central Brazil formed a polyphyletic cluster in the tree, indicating that these strains are similar to those from other geographic regions. These results contribute to the understanding of HIV in Brazil, and may prove useful for the development of vaccine candidates.
机译：这项研究分析了pol和env基因，以确定巴西中部HIV-1的遗传变异性。 41个感染HIV-1的个体分离株具有蛋白酶，逆转录酶和C2C3 / env，可通过巢式PCR扩增并测序。通过程序REGA和系统发育分析确定亚型。通过SimPlot分析鉴定为推定重组形式的样品。观察到B型的高流行（95.1％），其次是花叶病毒B / F（4.9％）。分析了来自30个HIV-1分离株的氨基酸序列的抗原性亚型多样性。最普遍的gp120 V3环基序是GPGR（美国/欧洲）（43.3％），以B和F亚型描述，其次是GPGK四肽（10％）。在6.7％的样本中发现了巴西变体B“（GWGR），GFGR和GLGR四肽。在八个分离株中也发现了其他V3变体（26.7％）。还进行了系统树分析以验证HIV-1的关系。来自巴西中部的样本以及在巴西流行的其他HIV-1序列，来自巴西中部的B亚型序列在树上形成了一个多系群，表明这些菌株与其他地理区域的菌株相似，这些结果有助于人们进一步了解巴西的艾滋病毒，可能对开发候选疫苗有用。

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