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首页> 外文期刊>Ticks and tick-borne diseases >Tissue localization of Coxiella-like endosymbionts in three European tick species through fluorescence in situ hybridization
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Tissue localization of Coxiella-like endosymbionts in three European tick species through fluorescence in situ hybridization

机译：三种欧洲蜱芯片在原位杂交中的三种欧洲蜱型物种中的Coxiella样谱的组织定位

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摘要

Ticks are commonly infected by Coxiella-like endosymbionts (Coxiella-LE) which are thought to supply missing B vitamin nutrients required for blood digestion.While this nutritional symbiosis is essential for the survival and reproduction of infected tick species, our knowledge of where Coxiella-LE is localized in tick tissues is partial at best since previous studies have focused on a limited number of Asian or American tick species. To fill this gap, we investigated the tissue localization of Coxiella-LE in three European tick species, Ornithodoros maritimus, Dermacentor marginatus and Ixodes hexagonus, using a diagnostic fluorescence in situ hybridization (FISH) assay, combined with PCR-based detection. Specific fluorescent foci were observed in several tick tissues. We visualized a pronounced tissue tropism of Coxiella-LE for tick ovaries and Malpighian tubules, a pattern suggestive of a high degree of lifestyle specialization toward mutualism: infection of the ovaries is indicative of transovarial transmission, whereas infection of the Malpighian tubules suggests a nutritional function. We postulate that Malpighian tubules are key organs for the nutritional symbiosis, notably the synthesis of B vitamins by Coxiella-LE, whereas the infection of the ovaries ensures vertical transmission of the symbionts to future generations. We also detected occasional infections in other organs, such as salivary glands and the midgut. Finally, we discuss the potential significance of the different tissue tropism for tick biology.
机译：蜱通常感染了Coxiella ideosymbionts（Coxiella-Le），被认为是供应血液消化所需的缺失的B维生素营养素。这种营养共生对于感染的蜱型物种的生存和繁殖至关重要，我们对Coxiella的知识 - Le在蜱组织中本地化是偏好的，因为之前的研究重点关注有限数量的亚洲或美国蜱型物种。为了填补这种差距，我们研究了三个欧洲蜱虫，奥纳西氏菌菌，奥纳西氏菌菌，Dermacentor Marginatus和Ixodes六甲醚的组织定位，使用原位杂交（鱼类）测定的诊断荧光，与基于PCR的检测相结合。在几种蜱组织中观察到特异性荧光灶。我们可视化Coxiella-Le的明显组织覆盖蜱卵巢和Malpighian小管，一种模式暗示了高度的生活方式朝着相互主义的专业化：卵巢的感染表明跨移动变速器，而Malpighian小管的感染表明营养功能。我们假设Malpighian小管是营养共生的关键器官，特别是Coxiella-Le的合成B族维生素，而卵巢的感染确保了对混合剂的垂直传播到后代。我们还发现其他器官的偶尔感染，例如唾液腺和中肠。最后，我们讨论了不同组织矫正蜱生物学的潜在意义。

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