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首页> 外文期刊>AIDS Research and Human Retroviruses >Short communication: Absence of evidence of HTLV-3 and HTLV-4 in patients with large granular lymphocyte (LGL) leukemia.
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Short communication: Absence of evidence of HTLV-3 and HTLV-4 in patients with large granular lymphocyte (LGL) leukemia.

机译：简短交流：大颗粒淋巴细胞（LGL）白血病患者缺乏HTLV-3和HTLV-4的证据。

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摘要

Clonal disorders of large granular lymphocytes (LGL) result in leukemia due to the expansion of a discrete subset of either CD3(+) T cells or natural killer (NK) cells. It has been hypothesized that a viral antigen acts as the initial stimulus causing the expansion of these cells. The possible involvement of human T cell lymphotropic virus types 1 and 2 (HTLV-1 and HTLV-2) in this disease has been studied but no conclusive evidence has linked either virus with LGL leukemia. In this study, we examined whether HTLV-3 or HTLV-4, two newly identified HTLV groups discovered in Central Africa in primate hunters, is involved in LGL leukemia. We developed two specific real-time PCR quantitative assays that are highly sensitive, capable of detecting 10 copies of HTLV-3 or HTLV-4 pol sequences in a background of 1 microg of DNA from human peripheral blood lymphocytes (PBL). We tested PBL DNA samples from 40 LGL leukemia patients in the United States and found that all samples were negative for HTLV-3 or HTLV-4 infection. These results suggest that HTLV-3 and HTLV-4 are not the causative agent of LGL leukemia.
机译：大颗粒淋巴细胞（LGL）的克隆性疾病会导致白血病，这是由于CD3（+）T细胞或自然杀伤（NK）细胞的离散子集的扩增所致。已经假设病毒抗原充当引起这些细胞扩增的初始刺激。已经研究了人类T细胞淋巴病毒1型和2型（HTLV-1和HTLV-2）可能参与该疾病，但尚无确凿证据将这两种病毒与LGL白血病联系起来。在这项研究中，我们检查了HTLV-3或HTLV-4，这是在中非的灵长类动物猎人中发现的两个新发现的HTLV组，是否与LGL白血病有关。我们开发了两种高度敏感的实时荧光定量PCR检测方法，能够在人外周血淋巴细胞（PBL）1微克DNA的背景下检测HTLV-3或HTLV-4 pol序列的10个拷贝。我们测试了来自美国40名LGL白血病患者的PBL DNA样本，发现所有样本的HTLV-3或HTLV-4感染均为阴性。这些结果表明HTLV-3和HTLV-4不是LGL白血病的病原体。

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