...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Toxicology Letters: An International Journal Providing a Forum for Original and Pertinent Contributions in Toxicology Research >Chlorophenols and chlorocatechols induce apoptosis in human lymphocytes (in vitro)
【24h】

Chlorophenols and chlorocatechols induce apoptosis in human lymphocytes (in vitro)

机译：氯酚和氯丁金诱导人淋巴细胞（体外）诱导细胞凋亡

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this work the effect of 2,4,5-trichlorophenol (2,4,5-TCP), pentachlorophenol (PCP), 4,6-dichloroguaiacol (4,6-DCG), tetrachloroguaiacol (TeCG), 4,5-dichlorocatechol (4,5-DCC) and tetrachlorocatechol (TeCC) on the induction of apoptosis in human peripheral blood lymphocytes was examined. The analysis of the changes in mitochondrial transmembrane potential was performed using JC-9 fluorescent probe. It was noted that all the compounds studied (excluding 4,6-DCG) in a dose-dependent manner (0.2-25 ppm) increased the number of cells, which were characterized by the reduction, with the strongest effect observed for TeCG and TeCC. Moreover, fluorimetric assay of the caspase-3 activity revealed that 2,4,5-TCP, chlorocatechols and TeCG in the range of the concentrations of 1-50 ppm increased the caspase-3 expression, whereas PCP in its highest concentration of 50 ppm decreased the activity of this enzyme. It was also noted that the caspase-3 activity was the most strongly induced by tetrachlorocatechols, particularly by TeCC. The analysis of YO-PRO-1 iodide/propidum iodide staining revealed that TeCC, the most strongly increased the number of apoptotic cells at 5 and 25 ppm, whereas at a dose of 100 ppm the strongest effect on apoptosis induction was observed for 2,4,5-TCP.
机译：在这项工作中，2,4,5-三氯苯酚（2,4,5-TCP），五氯苯酚（PCP），4,6-二氯胍（4,6-DCG），四氯胍（TECG），4,5-研究了二氯替氯替金属（4,5-DCC）和四氯咖啡（TECC）对人外周血淋巴细胞诱导凋亡的诱导。使用JC-9荧光探针进行线粒体跨膜电位变化的分析。注意，研究（不包括4,6-DCG）以剂量依赖性方式（0.2-25ppm）的所有化合物增加了细胞的数量，其特征在于减少，具有TECG和TECC的最强效应。此外，Caspase-3活性的荧光测定表明，在浓度为1-50ppm的浓度范围内的2,4,5-TCP，氯丁金和TECG增加了Caspase-3表达，而PCP的最高浓度为50ppm降低了这种酶的活性。还有人注意到，Caspase-3活性是四氯脲，特别是TECC最强烈的诱导。 yo-pro-1碘化物/碘化物染色的分析显示，TECC，最强烈地增加了5和25ppm的凋亡细胞数，而在100ppm的剂量下观察到2， 4,5-TCP。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号