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首页> 外文期刊>Toxicology Letters: An International Journal Providing a Forum for Original and Pertinent Contributions in Toxicology Research >Benzo(a)pyrene promotes migration, invasion and metastasis of lung adenocarcinoma cells by upregulating TGIF
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Benzo(a)pyrene promotes migration, invasion and metastasis of lung adenocarcinoma cells by upregulating TGIF

机译：通过上调TGIF来促进肺腺癌细胞的迁移，侵袭和转移，促进肺腺癌细胞的迁移，侵袭和转移

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This study aimed to investigate the potential roles of TG-interacting factor (TGIF) in benzo(a)pyrene (BaP)-induced migration, invasion, and metastasis of lung adenocarcinoma cells. Cells were treated with different concentrations of BaP. MTT assays were used to measure cell proliferation. Quantitative real-time polymerase chain reaction (qRT-PCR) and immunoblots were applied to measure the TGIF expression. A dual-luciferase reporter gene assay was performed to assess the effects of BaP on TGIF promoter-driven reporter gene expression. Wound-healing, transwell, and tail vein metastasis assays were performed to evaluate migratory, invasive, and metastatic capacity. Our results showed that BaP treatment increased the expression of TGIF mRNA and protein. Additionally, BaP treatment enhanced TGIF promoter-driven reporter gene expression. We observed that BaP treatment promoted the migration, invasion, and metastasis of H157 cells, which could be blocked by silencing TGIF. The expression of TGIF mRNA was significantly higher in metastatic lung adenocarcinoma samples than in non-metastatic lung adenocarcinoma samples, and higher levels of TGIF mRNA expression were observed in metastatic lung adenocarcinoma samples from patients with a smoking history than in those from patients with a non-smoking history. Our findings suggest that BaP treatment promotes the migration, invasion, and metastasis of human lung adenocarcinoma cells by upregulating TGIF.
机译：本研究旨在探讨TG相互作用因子（TGIF）在苯并（A）芘（BAP）诱导的迁移，侵袭和转移中的潜在作用。用不同浓度的壳体处理细胞。 MTT测定用于测量细胞增殖。施加定量实时聚合酶链反应（QRT-PCR）和免疫印迹以测量TGIF表达。进行双荧光素酶报告基因测定以评估BAP对TGIF启动子驱动的报告基因表达的影响。进行伤口愈合，Transwell和尾静脉转移测定以评估迁移，侵袭性和转移能力。我们的研究结果表明，BAP治疗增加了TGIF mRNA和蛋白质的表达。另外，BAP处理增强了TGIF启动子推动的报告基因表达。我们观察到，Bap治疗促进H157细胞的迁移，侵袭和转移，这可以通过沉默的TGIF阻止。转移性肺腺癌样品中TGIF mRNA的表达显着高于非转移性肺腺癌样品，并且在吸烟历史患者的转移性肺腺癌样本中观察到较高水平的TGIF mRNA表达，而不是非患者 - 纪念历史。我们的研究结果表明，通过上调TGIF来促进人肺腺癌细胞的迁移，侵袭和转移促进人肺腺癌细胞的迁移，侵袭和转移。

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