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首页> 外文期刊>Toxicology: An International Journal Concerned with the Effects of Chemicals on Living Systems >Exploration of the establishment of manganese poisoning rat model and analysis of discriminant methods
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Exploration of the establishment of manganese poisoning rat model and analysis of discriminant methods

机译：探讨锰中毒大鼠模型的建立及判别方法分析

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ObjectiveWe explored methods to establish an animal model of manganese poisoning and evaluate the feasibility of the determination method. MethodsTwenty-four specific pathogen-free male rats were randomly divided into four groups: control, low-dose (15.0?mg/kg), middle-dose (25.0?mg/kg), and high-dose (50.0?mg/kg). Intraperitoneal injection of MnCl2·H2O was administered every 48?h for three months. Rats were tested for behavior, muscle tension, and with a balance beam experiment at the end of each month. Three months later, the rats were sacrificed and brain tyrosine hydroxylase (TH) expression levels were measured. ResultsRats in each group exhibited changes in behavior, muscle tone, and balance after exposure to manganese, and the scores of each test for the high-dose and middle-dose groups were statistically different from the low-dose and control groups. Finally, a rat model of manganese poisoning was identified with the TH expression less than 30% of the normal value. We find that the modeling success rate of the middle-dose and high-dose groups were 66.67% and 100%, respectively. In addition, there were negative correlations between the three assessment methods such as behavioral tests and TH expression levels. ConclusionsIntraperitoneal injection of MnCl2·H2O (25?mg/kg) can successfully establish a manganese poisoning rat model with low mortality rate. Muscle tension, balance beam, and behavioral tests can be used as preliminary determination methods for modeling.
机译：目的探索锰中毒动物模型的探索方法，评价测定方法的可行性。方法将四种特异性病原体的无菌雄性大鼠随机分为四组：对照，低剂量（15.0×mg / kg），中剂量（25.0×mg / kg）和高剂量（50.0×mg / kg ）。每48μl施用腹膜内注射MnCl2·H 2 O 3个月。对行为，肌肉张力和每月末的平衡光束实验进行了测试。三个月后，处死大鼠，并测量大鼠脑酪氨酸羟化酶（TH）表达水平。在接触锰后，每组的结果表现出行为，肌肉色调和平衡的变化，并且高剂量和中剂量基团的每种测试的分数与低剂量和对照组统计学不同。最后，鉴定了锰中毒的大鼠模型，鉴定了少于正常值的30％。我们发现中剂量和高剂量组的建模成功率分别为66.67％和100％。此外，三种评估方法之间存在负相关性，例如行为测试和表达水平。结论InsionsInterRaproAneal注入MnCl2·H 2 O（25×mg / kg）可以成功地建立低死亡率的锰中毒大鼠模型。肌肉张力，平衡梁和行为试验可用作建模的初步测定方法。

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