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首页> 外文期刊>AIDS Research and Human Retroviruses >Full-length HIV type 1 proviral sequencing of 10 highly exposed women from Nairobi, Kenya reveals a high proportion of intersubtype recombinants.
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Full-length HIV type 1 proviral sequencing of 10 highly exposed women from Nairobi, Kenya reveals a high proportion of intersubtype recombinants.

机译：肯尼亚内罗毕的10位高暴露女性的全长HIV 1型前病毒测序表明亚型重组体比例很高。

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Phylogenetic analysis has revealed that the current HIV/AIDS pandemic consists of a multitude of different viral clades and recombinant viruses. The predominant circulating HIV-1 clade in Kenya is A1; however, Kenya borders countries where different subtypes are prominent, making Kenya a likely location for recombination. Previous studies have reported significant differences in the proportions of sequences in Kenya that are intersubtype recombinants. Studies that performed sequence-based typing on multiple HIV-1 genomic regions or full-length sequences found higher rates of recombination than those that examined a single gene or gene fragment. In this study, we describe full-length HIV-1 proviral sequence-based genotyping after limited peripheral blood mononuclear cell (PBMC) coculture. Ten subjects from a highly exposed cohort located in Nairobi, Kenya were examined. Pairwise comparison found minimal difference between sequences generated directly from patient PBMC DNA compared to sequences from cocultured PBMC DNA. Of the 10 full-length HIV-1 sequences examined, five were nonrecombinant clade A1, while the other five were unique intersubtype recombinants. Although this frequency of recombination is higher than previously described in Kenya, this finding is in agreement with previous full-length sequence data. Interestingly, although all the nonrecombinant sequences were clade A1, not all the recombinant sequences contained a clade A1 sequence.
机译：系统发育分析表明，当前的艾滋病毒/艾滋病大流行由多种不同的病毒进化枝和重组病毒组成。肯尼亚主要的循环HIV-1进化枝为A1;但是，肯尼亚与不同亚型的国家接壤，因此肯尼亚很可能成为重组地点。先前的研究报道了肯尼亚亚型间重组子序列比例的显着差异。在多个HIV-1基因组区域或全长序列上进行基于序列的分型的研究发现，重组率要高于检查单个基因或基因片段的重组率。在这项研究中，我们描述了有限的外周血单核细胞（PBMC）共培养后的全长基于HIV-1前病毒序列的基因分型。来自肯尼亚内罗毕的一个高度暴露人群的十名受试者接受了检查。配对比较发现，直接从患者PBMC DNA产生的序列与共培养PBMC DNA的序列之间的差异最小。在检查的10条全长HIV-1序列中，有5条是非重组进化枝A1，而其他5条是独特的亚型重组体。尽管这种重组频率高于肯尼亚先前的描述，但这一发现与先前的全长序列数据相符。有趣的是，尽管所有非重组序列均为进化枝A1，但并非所有重组序列均含有进化枝A1序列。

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