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首页> 外文期刊>Transgenic research >Overexpression of the maize gamma-tocopherol methyltransferase gene (ZmTMT) increases alpha-tocopherol content in transgenic Arabidopsis and maize seeds
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Overexpression of the maize gamma-tocopherol methyltransferase gene (ZmTMT) increases alpha-tocopherol content in transgenic Arabidopsis and maize seeds

机译：玉米γ-生育酚甲基转移酶基因（ZMTMT）的过表达增加了转基因拟南芥和玉米种子中的α-生育酚含量

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摘要

The vitamin E family includes tocopherols and tocotrienols, which are essential lipid-soluble antioxidants necessary for human and livestock health. The seeds of many plant species, including maize, have high gamma (gamma)-tocopherol but low alpha (alpha)-tocopherol contents; however, alpha-tocopherol is the most effective antioxidant. Therefore, it is necessary to optimize the tocopherol composition in plants. alpha-Tocopherol is synthesized from gamma-tocopherol by gamma-tocopherol methyltransferase (gamma-TMT, VTE4) in the final step of the tocopherol biosynthetic pathway. In the present study, the full-length coding sequence (CDS) of gamma-TMT was isolated from Zea mays, named ZmTMT. The ZmTMT CDS was 1059 bp in size, encoding 352 amino acids. Recombinant ZmTMT was expressed in Escherichia coli and the purified protein effectively converted gamma-tocopherol into alpha-tocopherol in vitro. A comparison of enzyme activities showed that the activity of ZmTMT was higher than that of GmTMT2a (Glycine max) and AtTMT (Arabidopsis thaliana). Overexpression of ZmTMT increased the alpha-tocopherol content 4-5-fold in transgenic Arabidopsis and around 6.5-fold in transgenic maize kernels, and increased the alpha-/gamma-tocopherol ratio to approximately 15 and 17, respectively. These results show that it is feasible to overexpress ZmTMT to optimize the tocopherol composition in maize; such a corn product might be useful in the feed industry in the near future.
机译：维生素E家族包括生育酚和Tocotrienols，这是人类和牲畜健康所必需的必需脂质可溶性抗氧化剂。许多植物种类的种子，包括玉米，具有高γ（γ） - ob，但低α（alpha） - 托酚含量;然而，α-生育酚是最有效的抗氧化剂。因此，有必要优化植物中的生育酚组合物。在生育酚生物合成途径的最后步骤中，通过γ-生育酚甲基转移酶（Gamma-TMT，VTE4）从γ-生育酚（Gamma-TMT，VTE4）合成α-生育酚。在本研究中，从Zea Mays中分离出γ-TMT的全长编码序列（CDS），命名为ZMTMT。 ZMTMT CD的大小为1059bp，编码352个氨基酸。重组ZMTMT在大肠杆菌中表达，纯化的蛋白质有效地将γ-生育酚转化为体外α-生育酚。酶活性的比较表明，ZMTMT的活性高于GMTMT2A（甘氨酸MAX）和attmt（拟南芥）的活性。 ZMTMT的过度表达在转基因拟南芥中增加了α-生育酚含量4-5倍，在转基因玉米核中约为6.5倍，并分别增加了α/γ-生育酚比至约15和17。这些结果表明，过度表达ZMTMT是可行的，以优化玉米中的生育酚组成;这种玉米产品在不久的将来可以在饲料行业中有用。

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  • 来源
    《Transgenic research》 |2020年第1期|共10页
  • 作者

    Zhang Lan; Luo Yanzhong; Liu Bin; Zhang Liang; Zhang Wei; Chen Rumei; Wang Lei;

  • 作者单位

    Chinese Acad Agr Sci Biotechnol Res Inst Natl Key Facil Crop Gene Resources &

    Genet Improv Beijing 100081 Peoples R China;

    Chinese Acad Agr Sci Biotechnol Res Inst Natl Key Facil Crop Gene Resources &

    Genet Improv Beijing 100081 Peoples R China;

    Chinese Acad Agr Sci Biotechnol Res Inst Natl Key Facil Crop Gene Resources &

    Genet Improv Beijing 100081 Peoples R China;

    Chinese Acad Agr Sci Biotechnol Res Inst Natl Key Facil Crop Gene Resources &

    Genet Improv Beijing 100081 Peoples R China;

    Chinese Acad Agr Sci Biotechnol Res Inst Natl Key Facil Crop Gene Resources &

    Genet Improv Beijing 100081 Peoples R China;

    Chinese Acad Agr Sci Biotechnol Res Inst Natl Key Facil Crop Gene Resources &

    Genet Improv Beijing 100081 Peoples R China;

    Chinese Acad Agr Sci Biotechnol Res Inst Natl Key Facil Crop Gene Resources &

    Genet Improv Beijing 100081 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医学遗传学;
  • 关键词

    alpha-Tocopherol; alpha-; gamma-Tocopherol ratio; gamma-Tocopherol methyltransferase (gamma-TMT); Zea mays;

    机译：α-生育酚;α-;γ-生育酚比例;γ-生育酚甲基转移酶（γ-tmt）;Zea Mays;

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